Human SERPINB5 (MASPIN) knockout HeLa cell lysate
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SERPINB5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
View Alternative Names
PI-5, Peptidase inhibitor 5, Protease inhibitor 5, SPB5_HUMAN, Serine (or cysteine) peptidase inhibitor, clade B, member 5, Serpin B5, Serpin peptidase inhibitor, Serpin peptidase inhibitor, clade B (ovalbumin), member 5, Spi5, Spi7, protease inhibitor 5 (maspin), serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 5, serpin family B member 5
- WB
Lab
Western blot - Human SERPINB5 (MASPIN) knockout HeLa cell lysate (AB258659)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SERPINB5 knockout HeLa cell lysate 20 μg
Lane 3 : HaCaT cell lysate 20 μg
Lane 4 : Caco-2 cell lysate 20 μg
False colour image of Western blot : Anti-MASPIN antibody (ab65136) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab65136 was shown to bind specifically to MASPIN. A band was observed at 42 kDa in wild-type HeLa cell lysates with no signal observed at this size in SERPINB5 knockout cell line ab264750 (knockout cell lysate ab258659). To generate this image, wild-type and SERPINB5 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-MASPIN antibody (<a href='/en-us/products/primary-antibodies/maspin-antibody-ab65136'>ab65136</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SERPINB5 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SERPINB5 (MASPIN) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-serpinb5-maspin-knockout-hela-cell-line-ab264750'>ab264750</a>)
Lane 3:
HaCaT cell lysate at 20 µg
Lane 4:
Caco-2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
false
- WB
Lab
Western blot - Human SERPINB5 (MASPIN) knockout HeLa cell lysate (AB258659)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SERPINB5 knockout HeLa cell lysate 20 μg
Lane 3 : HaCaT cell lysate 20 μg
Lane 4 : Caco-2 cell lysate 20 μg
False colour image of Western blot : Anti-MASPIN antibody (ab272858) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab272858 was shown to bind specifically to MASPIN. A band was observed at 42 kDa in wild-type HeLa cell lysates with no signal observed at this size in SERPINB5 knockout cell line ab264750 (knockout cell lysate ab258659). To generate this image, wild-type and SERPINB5 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-MASPIN antibody (<a href='/en-us/products/primary-antibodies/maspin-antibody-ab272858'>ab272858</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SERPINB5 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SERPINB5 (MASPIN) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-serpinb5-maspin-knockout-hela-cell-line-ab264750'>ab264750</a>)
Lane 3:
HaCaT cell lysate at 20 µg
Lane 4:
Caco-2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
false
- WB
Lab
Western blot - Human SERPINB5 (MASPIN) knockout HeLa cell lysate (AB258659)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SERPINB5 knockout HeLa cell lysate 20 μg
Lane 3 : HaCaT cell lysate 20 μg
Lane 4 : Caco-2 cell lysate 20 μg
False colour image of Western blot : Anti-MASPIN antibody (ab182785) staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab182785 was shown to bind specifically to MASPIN. A band was observed at 42 kDa in wild-type HeLa cell lysates with no signal observed at this size in SERPINB5 knockout cell line ab264750 (knockout cell lysate ab258659). To generate this image, wild-type and SERPINB5 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-MASPIN antibody (<a href='/en-us/products/primary-antibodies/maspin-antibody-ab182785'>ab182785</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SERPINB5 knockout HeLa cell lysate at 20 µg
Lane 3:
HaCaT cell lysate at 20 µg
Lane 4:
Caco-2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SERPINB5 (MASPIN) knockout HeLa cell lysate (AB258659)
Homozygous : 1 bp insertion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
MASPIN influences apoptosis and cell motility playing a critical role in tumor suppression. It does not form part of a complex but interacts directly with extracellular matrix components. MASPIN can inhibit angiogenesis by reducing the proliferation and migration of endothelial cells. Scientists have noted its role in maintaining normal cell architecture and inhibiting tumor cell invasion.
Pathways
Researchers place MASPIN in important signaling networks impacting cancer progression and metastasis. It participates in the PI3K/AKT pathway thereby influencing cell growth and survival. MASPIN also intersects with TGF-β signaling and related proteins like SMAD which mediate growth arrest and apoptosis. By altering these pathways MASPIN helps modulate responses to cellular stress and inhibit lethal growth in cancer cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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