Human SERTAD3 knockout HeLa cell lysate
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SERTAD3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
AW124805, D1Mgi51, MGC108974, RPA-binding trans-activator, Rbt1, Replication protein-binding trans-activator, SERTA domain-containing protein 3, SERTAD3, SRTD3_HUMAN, Sei3
- Sanger seq
Unknown
Sanger Sequencing - Human SERTAD3 knockout HeLa cell lysate (AB258660)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SERTAD3 knockout HeLa cell lysate (AB258660)
Allele-1 : 17 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SERTAD3 plays a role in cellular growth and division processes. As a part of multi-protein complexes it interacts with cyclin-dependent kinase 4 (CDK4) and cyclin D1. This interaction helps regulate the cell cycle particularly influencing the transition from the G1 to the S phase. SERTAD3 contributes significantly to controlling cell division making it important for maintaining normal cellular homeostasis.
Pathways
Several aspects of cell cycle regulation involve SERTAD3. It is an important participant in the cyclin D-CDK4/6 signaling pathway which is necessary for cell cycle progression. SERTAD3 interacts with retinoblastoma protein (pRB) through this pathway impacting its phosphorylation status and thereby influencing cell cycle control. The interaction helps facilitate proper DNA synthesis and replication.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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