Human SESN2 (Sestrin-2) knockout HEK-293 cell lysate
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SESN2 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 1 bp deletion Frameshift = 99.77%.
View Alternative Names
DKFZp761M0212, DKFZp761M02121, Hi95, Hypoxia induced gene 95, MGC11758, OTTHUMP00000003641, OTTMUSP00000010184, RP23 63D8.3, SES2, SESN2_HUMAN, SEST2, Sestrin-2
- WB
Lab
Western blot - Human SESN2 (Sestrin-2) knockout HEK-293 cell lysate (AB269650)
Lane 1 : Wild-type HEK-293 cell lysate 20 μg
Lane 2 : SESN2 knockout HEK-293 cell lysate 20 μg
False colour image of Western blot : Anti-SESN2/Sestrin-2 antibody [EPR18907] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab178518 was shown to bind specifically to SESN2/Sestrin-2. A band was observed at 54 kDa in wild-type HEK-293 cell lysates with no signal observed at this size in SESN2 knockout cell line ab269486 (knockout cell lysate ab269650). To generate this image, wild-type and SESN2 knockout HEK-293 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-SESN2/Sestrin-2 antibody [EPR18907] (<a href='/en-us/products/primary-antibodies/sesn2-sestrin-2-antibody-epr18907-ab178518'>ab178518</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293 cell lysate at 20 µg
Lane 2:
SESN2 knockout HEK-293 cell lysate at 20 µg
Lane 2:
Western blot - Human SESN2 (Sestrin-2) knockout HEK-293 cell line (<a href='/en-us/products/cell-lines/human-sesn2-sestrin-2-knockout-hek-293-cell-line-ab269486'>ab269486</a>)
Predicted band size: 54 kDa
Observed band size: 54 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Sestrin-2 regulates cell survival and homeostasis during oxidative stress conditions. It modulates the activity of key signaling pathways that control autophagy and cell growth. Sestrin-2 does not function alone but interacts with other proteins to form functional complexes. This allows it to fine-tune cellular processes and contributes to its role in promoting cell repair mechanisms. Its induction during stress conditions demonstrates its engagement in promoting cellular adaptation and resistance to damage.
Pathways
Sestrin-2 engages in signaling networks such as the AMPK and mTOR pathways. Its interaction with AMP-activated protein kinase (AMPK) triggers energy homeostasis and metabolic stress responses. It also negatively regulates the mammalian target of rapamycin (mTOR) to support autophagy and inhibit cell growth when necessary. Sestrin-2 therefore plays an important role in coordinating responses between these pathways ensuring optimal cellular function during periods of environmental stress or nutrient deprivation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB178518
Anti-SESN2/Sestrin-2 antibody [EPR18907]
RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-SESN2/Sestrin-2 antibody [EPR18907] (AB178518)](https://content.abcam.com/products/images/sesn2-sestrin-2-antibody-epr18907-ab178518--immunocytochemistry-immunofluorescence-img62583.jpg)
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Cell Lines & Lysates
AB269486
Human SESN2 (Sestrin-2) knockout HEK-293 cell line
Advanced Validation
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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