Human SETD2 (KMT3A / HYPB / HIF-1) knockout HeLa cell lysate
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- WB
Lab
Western blot - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HeLa cell lysate (AB257273)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SETD2 knockout HeLa cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : Human Heart cell lysate 20 μg
False colour image of Western blot : Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] staining at 1/1000 dilution, shown in black; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab239350 was shown to bind specifically to KMT3A / HYPB / HIF-1. A band was observed at 120-280, 440 kDa in wild-type HeLa cell lysates with no signal observed at this size in SETD2 knockout cell line ab261792 (knockout cell lysate ab257273). To generate this image, wild-type and SETD2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (<a href='/en-us/products/primary-antibodies/kmt3a-hypb-hif-1-antibody-epr20927-67-ab239350'>ab239350</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SETD2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-setd2-kmt3a-hypb-hif-1-knockout-hela-cell-line-ab261792'>ab261792</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
Human Heart cell lysate at 20 µg
Predicted band size: 288 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HeLa cell lysate (AB257273)
Allele-2 : 1 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SETD2 (KMT3A / HYPB / HIF-1) knockout HeLa cell lysate (AB257273)
Allele-1 : 2 bp deletion in exon3
Complementary Products
Primary Antibodies
AB239350
Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67]
RabMAb|Recombinant|KO Validated|Trial Size|Trial Size
![Western blot - Anti-KMT3A / HYPB / HIF-1 antibody [EPR20927-67] (AB239350)](https://content.abcam.com/products/images/kmt3a-hypb-hif-1-antibody-epr20927-67-ab239350--western-blot-img167197.jpg)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
KMT3A modifies chromatin structure to regulate gene expression. Its histone methylation function plays a critical role in the modulation of transcriptional activity ensuring that genes are turned on or off when necessary for cellular function. Additionally KMT3A forms part of complex protein networks that mediate gene expression changes in response to cellular signals. The precise regulation of this activity is vital for maintaining normal cellular function and responding to environmental cues.
Pathways
KMT3A engages in significant pathways such as the transcriptional regulation and epigenetic modification pathways. It interacts with various proteins including CoREST and LSD1 within these pathways to modulate gene expression and influence cellular differentiation. Through these interactions KMT3A ensures the correct genetic programs are executed aligning with developmental and homeostatic requirements.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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