Human SFN (14-3-3 sigma) knockout HeLa cell lysate
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SFN KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1.
View Alternative Names
14-3-3 protein sigma, 1433S_HUMAN, ER, Epithelial cell marker protein 1, MGC143283, Mme1, OTTHUMP00000004242, RP23 137L22.11, SFN, SFN protein, Stratifin, YWHAS
- WB
Unknown
Western blot - Human SFN (14-3-3 sigma) knockout HeLa cell lysate (AB257288)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : SFN knockout HeLa cell lysate (20 μg)
Lane 3 : A431 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab14123 observed at 28 kDa. Red - loading control, ab52866 observed at 50 kDa.
ab14123 Anti-14-3-3 sigma/SFN antibody [1433S01] was shown to specifically react with 14-3-3 sigma/SFN in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261779 (knockout cell lysate ab257288) was used. Wild-type and 14-3-3 sigma/SFN knockout samples were subjected to SDS-PAGE. ab14123 and Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (ab52866) were incubated overnight at 4°C at 1 in 200 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Anti-14-3-3 sigma/SFN antibody [1433S01] (<a href='/en-us/products/unavailable/14-3-3-sigmasfn-antibody-1433s01-ab14123'>ab14123</a>) at 1/200 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SFN knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SFN (14-3-3 sigma) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-sfn-14-3-3-sigma-knockout-hela-cell-line-ab261779'>ab261779</a>)
Lane 3:
A431 cell lysate at 20 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human SFN (14-3-3 sigma) knockout HeLa cell lysate (AB257288)
Allele-1 : 2 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SFN (14-3-3 sigma) knockout HeLa cell lysate (AB257288)
Allele-2 : 1 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
14-3-3 sigma contributes to cell cycle arrest and apoptosis. As part of a complex it interacts with other proteins to ensure proper cell division and prevent abnormal growth. It binds to phosphorylated serine/threonine residues on its target proteins influencing their activity and function. By doing this it exerts control over cell cycle checkpoints and influences DNA damage response mechanisms.
Pathways
14-3-3 sigma interacts with several pathways that are critical for maintaining cell stability and response to stress. It participates in the PI3K/AKT pathway working closely with proteins like AKT1 to facilitate cell survival and growth suppression under stress conditions. Its involvement in this pathway ties it to the regulation of cell proliferation and survival interconnecting with broader cellular networks.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Primary Antibodies
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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