Human SFRP1 knockout A549 cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
SFRP1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Homozygous CRISPR/Cas9 KO: the 52 bp deletion in exon 2 leads to a truncated SFRP1 (tSFRP1) rather than a complete loss of protein. tSFRP1 is not secreted out of the cell, in contrast with WT SFRP1.
View Alternative Names
FRP, FRP-1, Frizzled related protein 1, FrzA, SARP-2, SFRP1_HUMAN, Secreted apoptosis-related protein 2, Secreted frizzled related protein, Secreted frizzled-related protein 1
- Sanger seq
Supplier Data
Sanger Sequencing - Human SFRP1 knockout A549 cell lysate (AB283831)
52 bp deletion in exon 2, lead to premature stop codon
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The secreted glycoprotein SFRP1 plays a role in cellular processes such as cell growth differentiation and apoptosis. As a member of the soluble frizzled-related proteins it acts to inhibit Wnt signaling pathways which are critical in maintaining cellular homeostasis. SFRP1 commonly interacts in the extracellular environment where it forms part of a regulatory complex that includes other extracellular matrix components to achieve its function.
Pathways
The role SFRP1 plays is important in the Wnt signaling pathway and the regulation of β-catenin activity. These pathways are involved in embryonic development and tissue regeneration. SFRP1 directly interacts with other proteins like frizzled receptors and Wnt ligands to maintain balance in signaling. This modulation further influences gene expression patterns and cellular behavior.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
![Western blot - Anti-SFRP1 antibody [EPR7003] (AB126613)](https://content.abcam.com/products/images/sfrp1-antibody-epr7003-ab126613--western-blot-img429979.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com