Human SH2D4A knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SH2D4A knockout HeLa cell lysate (AB259108)
Allele-1 : 5 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SH2D4A knockout HeLa cell lysate (AB259108)
Allele-2 : 1 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SH2D4A protein participates in the regulation of immune responses. It is not considered part of a large complex but interacts with other signaling molecules to influence downstream signaling cascades. By engaging with phosphorylated partners SH2D4A modulates pathways important for cell growth proliferation and immune activation. This function positions SH2D4A as an important mediator in cellular signaling networks underpinning immune system function.
Pathways
Research identifies SH2D4A as influencing both the MAPK and JAK-STAT pathways. It interacts with key proteins within these pathways such as MAPK1 which is integral for transmitting extracellular signals into cellular responses. These pathways regulate processes essential for cell division growth and development. SH2D4A's interaction with signaling components supports its role in controlling cellular responses to external stimuli and maintaining cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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