Human SH3BGRL3 knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
SH3BGRL3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
HEL-S-297, SH3 domain binding glutamic acid rich protein like 3, SH3 domain-binding glutamic acid-rich-like protein 3, SH3 domain-binding protein 1, SH3BGRL3 like protein, SH3L3_HUMAN, TIP-B1, TNF inhibitory protein, epididymis secretory protein Li 297
- Sanger seq
Unknown
Sanger Sequencing - Human SH3BGRL3 knockout HEK-293T cell lysate (AB258661)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein is involved in several cellular processes including signal transduction and cytoskeleton organization. SH3BGRL3 functions as part of a protein complex that modulates interactions between signaling molecules and cytoskeletal components. It plays an important role in maintaining cellular architecture and facilitating cell communication by interacting with other proteins inside the cell. This interaction can affect cell structure and growth.
Pathways
This protein participates in signal transduction and cytoskeletal dynamics pathways. It interacts with proteins such as Src which is involved in various signaling cascades. SH3BGRL3's connection to the Src signaling pathway is significant as it can modulate processes like cell proliferation and differentiation. The protein is also linked with actin cytoskeleton pathways further highlighting its role in maintaining cell structure integrity.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com