Human SHC2 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SHC2 knockout HeLa cell lysate (AB259110)
Allele-1 : 7 bp deletion in exon6
- Sanger seq
Unknown
Sanger Sequencing - Human SHC2 knockout HeLa cell lysate (AB259110)
Allele-2 : 1 bp insertion in exon6
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SHC2 plays significant roles in the regulation of signal transduction pathways that control cell growth apoptosis and differentiation. It participates in the formation of complexes that relay signals from activated receptor tyrosine kinases. SHC2 by binding to these receptors through its SH2 domain helps in the recruitment of downstream signaling molecules like GRB2 leading to the activation of signaling cascades.
Pathways
SHC2 is critically involved in the MAPK/ERK signaling pathway which transmits signals from the cell surface to the DNA in the cell nucleus. This pathway's activation ultimately leads to cell growth and differentiation processes. SHC2 interacts with proteins such as GRB2 and SOS fostering signal transduction that is essential for maintaining proper cellular functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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