Human SHC3 (SHCC) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SHC3 (SHCC) knockout HeLa cell lysate (AB259111)
Homozygous : 20 bp deletion in exon4
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SHC3 acts as a mediator in the signaling pathways that regulate cell proliferation differentiation and survival. It forms part of multi-protein complexes involved in transducing signals from the cell surface to the nucleus. Upon activation SHC3 interacts with various signaling proteins to propagate essential cellular signals that contribute to neuronal development and function. These interactions highlight its importance in maintaining cellular communication within neural environments.
Pathways
SHC3 is intricately involved in the MAPK/ERK pathway and PI3K/AKT pathway both critical for cell survival and growth. In the MAPK/ERK pathway SHC3 links receptors like Trk to downstream effectors facilitating signal transmission. It also interacts with important proteins such as Grb2 and Ras serving as a scaffold to support signaling cascades that regulate neural cell function. Through these pathways SHC3 plays an integral part in linking external cellular cues to fine-tune intracellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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