Human SHC4 (SHCD) knockout HeLa cell lysate
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SHC4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 4 bp deletion in exon4.
View Alternative Names
MGC34023, RaLP, Rai-like protein, SH2 domain protein C4, SHC (Src homology 2 domain containing) family member 4, SHC adaptor protein 4, SHC family member 4, SHC-transforming protein 4, SHC-transforming protein D, SHC4_HUMAN, SHCD, Src homology 2 domain containing family member 4, Src homology 2 domain-containing-transforming protein C4, hShcD
- Sanger seq
Unknown
Sanger Sequencing - Human SHC4 (SHCD) knockout HeLa cell lysate (AB259112)
Allele-1 : 4 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human SHC4 (SHCD) knockout HeLa cell lysate (AB259112)
Allele-2 : 1 bp deletion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SHC4 serves as an adaptor protein that connects activated receptors to downstream signaling pathways. It can associate with various signaling complexes helping to mediate responses to extracellular stimuli. SHC4 transmits signals from receptors like receptor tyrosine kinases (RTKs) and plays a part in cellular functions such as proliferation and differentiation. These attributes highlight its involvement in complex networks that control cellular responses.
Pathways
SHC4 participates in signaling cascades like the MAPK/ERK and PI3K/AKT pathways. It interacts with proteins such as GRB2 which transmits signals from activated RTKs to the Ras protein ultimately influencing cell fate decisions. These pathways are critical for regulating cell cycle progression cellular growth and metabolism demonstrating SHC4's integral function in these processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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