Human SIDT1 knockout A549 cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SIDT1 knockout A549 cell lysate (AB258665)
Homozygous : 1 bp insertion in exon11
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SIDT1 is involved in the RNA interference (RNAi) pathway which regulates gene expression. This protein is not known to form parts of larger protein complexes but serves as a channel for dsRNA molecules. SIDT1's activity can affect gene silencing processes by facilitating the entry of siRNA from the extracellular environment into the cell cytoplasm playing an important role in post-transcriptional gene regulation.
Pathways
SIDT1 plays a role in the RNA interference pathway important for post-transcriptional regulation of genes. It is associated with the machinery of RNAi which includes Dicer and Argonaute proteins. These interactions are important because they allow for precise gene expression control. SIDT1 facilitates the cellular uptake of siRNA thereby influencing gene expression levels and impacting regulatory pathways tied to cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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