Human SIKE1 knockout HeLa cell lysate
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- WB
Supplier Data
Western blot - Human SIKE1 knockout HeLa cell lysate (AB258188)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : SIKE1 knockout HeLa cell lysate (20 μg)
Lane 3 : HEK-293 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab183509 observed at 25 kDa. Red - loading control ab8245 observed at 37 kDa.
ab183509 Rabbit monoclonal [EPR14692] to SIKE1 was shown to specifically react with Suppressor of IKBKE 1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265933 (knockout cell lysate ab258188) was used. Wild-type and Suppressor of IKBKE 1 knockout samples were subjected to SDS-PAGE. ab183509 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SIKE1 antibody [EPR14692] (<a href='/en-us/products/primary-antibodies/sike1-antibody-epr14692-ab183509'>ab183509</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SIKE1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SIKE1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-sike1-knockout-hela-cell-line-ab265933'>ab265933</a>)
Lane 3:
HEK-293 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SIKE1 knockout HeLa cell lysate (AB258188)
Allele-1 : 1 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SIKE1 knockout HeLa cell lysate (AB258188)
Allele-2 : 1 bp insertion in exon2
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SIKE1 antibody [EPR14692] (AB183509)](https://content.abcam.com/products/images/sike1-antibody-epr14692-ab183509--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112046.jpg)
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SIKE1 interacts with signaling pathways to modulate immune responses. As a negative regulator of the interferon signaling pathway it forms complexes with TBK1/IKKε to suppress excessive immune activation preventing potential tissue damage. By efficiently regulating kinase activity SIKE1 helps maintain balance in immune signaling ensuring proper immune defense while avoiding overactivation.
Pathways
The involvement of SIKE1 extends to the toll-like receptor (TLR) and retinoic acid-inducible gene I (RIG-I)-like receptor signaling pathways. These pathways play essential roles in detecting and responding to viral pathogens. SIKE1 modulates the activity of TBK1 and IKKε which are central to the aforementioned pathways impacting downstream molecules like IRF3 and NF-kB that drive interferon production and inflammatory responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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