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SIRT6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 4 bp deletion in exon 1.

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Images

Western blot - Human SIRT6 knockout HeLa cell lysate (AB257673), expandable thumbnail
  • Western blot - Human SIRT6 knockout HeLa cell lysate (AB257673), expandable thumbnail
  • Sanger Sequencing - Human SIRT6 knockout HeLa cell lysate (AB257673), expandable thumbnail
  • Sanger Sequencing - Human SIRT6 knockout HeLa cell lysate (AB257673), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 4 bp deletion in exon 1.

Alternative names

2810449N18Rik, AI043036, Mono ADP ribosyltransferase sirtuin 6, NAD-dependent protein deacetylase sirtuin-6, Regulatory protein SIR2 homolog, Regulatory protein SIR2 homolog 6, SIR2 like 6, SIR2-like protein 6, SIR2L6, SIR6_HUMAN, Sir2 related protein type 6, Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae), Sirtuin 6, Sirtuin type 6

What's included?

1 Kit
Components
Human SIRT6 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

SIRT6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 4 bp deletion in exon 1.

Key facts

Cell type
HeLa
Mutation description
Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 4 bp deletion in exon 1.
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
SIRT6
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The SIRT6 protein also known as Sirtuin 6 plays an important role in cellular regulation. It is a member of the sirtuin family of proteins which are NAD+-dependent deacetylases. SIRT6 weighs approximately 39 kDa and can be found in the nucleus of many cell types. It acts mainly in regulating cellular homeostasis by removing acetyl groups from histone proteins which affects gene expression. Its expression levels vary across different tissues and it is known to be active in metabolically active organs such as the liver and brain.

Biological function summary

SIRT6 influences DNA repair metabolism and inflammation. It participates in maintaining genomic stability by promoting base excision repair a critical DNA repair process. Moreover SIRT6 contributes to glucose homeostasis by influencing gluconeogenesis and glycolysis. This protein is not known to be part of any larger protein complexes but it interacts individually with other proteins to exert its biological effects.

Pathways

SIRT6 plays a significant role in two key biological pathways: DNA damage response and metabolism regulation. In the DNA damage response pathway SIRT6 works with other proteins like PARP1 to facilitate DNA repair under stress conditions. In the regulation of metabolism SIRT6 interacts with transcription factors like HIF1α which influences the expression of genes involved in glycolytic metabolism and glucose homeostasis.

Associated diseases and disorders

SIRT6 has associations with cancer and aging-related diseases. SIRT6 has a protective effect against oncogenesis by maintaining genomic stability and regulating metabolic pathways that cancer cells exploit. Additionally its role in aging is connected to its ability to prevent age-related genomic instability and metabolic decline. In cancer SIRT6 interacts with proteins such as c-Myc and p53 influencing cell growth and apoptosis pathways. This highlights its potential as a therapeutic target for both cancer and age-related conditions.

Product promise

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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