Human SIRT6 knockout HeLa cell lysate
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SIRT6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and 4 bp deletion in exon 1.
View Alternative Names
2810449N18Rik, AI043036, Mono ADP ribosyltransferase sirtuin 6, NAD-dependent protein deacetylase sirtuin-6, Regulatory protein SIR2 homolog, Regulatory protein SIR2 homolog 6, SIR2 like 6, SIR2-like protein 6, SIR2L6, SIR6_HUMAN, Sir2 related protein type 6, Sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae), Sirtuin 6, Sirtuin type 6
- WB
Lab
Western blot - Human SIRT6 knockout HeLa cell lysate (AB257673)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : SIRT6 knockout HeLa cell lysate (20 μg)
Lanes 1-2 : Merged signal (red and green). Green - ab176345 observed at 45 kDa. Red - loading control ab8245 observed at 37 kDa.
ab176345 Anti-SIRT6 antibody [EPR5079(N)] was shown to specifically react with SIRT6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265054 (knockout cell lysate ab257673) was used. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE. ab176345 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SIRT6 antibody [EPR5079(N)] (<a href='/en-us/products/primary-antibodies/sirt6-antibody-epr5079n-ab176345'>ab176345</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SIRT6 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SIRT6 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-sirt6-knockout-hela-cell-line-ab265054'>ab265054</a>)
Predicted band size: 39 kDa
Observed band size: 45 kDa
false
- WB
Lab
Western blot - Human SIRT6 knockout HeLa cell lysate (AB257673)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : SIRT6 knockout HeLa cell lysate (20 μg)
Lanes 1-2 : Merged signal (red and green). Green - ab191385 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab191385 Anti-SIRT6 antibody [EPR18463] was shown to specifically react with SIRT6 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265054 (knockout cell lysate ab257673) was used. Wild-type and SIRT6 knockout samples were subjected to SDS-PAGE. ab191385 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 Dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SIRT6 antibody [EPR18463] (<a href='/en-us/products/primary-antibodies/sirt6-antibody-epr18463-ab191385'>ab191385</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SIRT6 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SIRT6 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-sirt6-knockout-hela-cell-line-ab265054'>ab265054</a>)
Predicted band size: 39 kDa
Observed band size: 40 kDa,42 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SIRT6 knockout HeLa cell lysate (AB257673)
Allele-2 : 2 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SIRT6 knockout HeLa cell lysate (AB257673)
Allele-1 : 4 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SIRT6 influences DNA repair metabolism and inflammation. It participates in maintaining genomic stability by promoting base excision repair a critical DNA repair process. Moreover SIRT6 contributes to glucose homeostasis by influencing gluconeogenesis and glycolysis. This protein is not known to be part of any larger protein complexes but it interacts individually with other proteins to exert its biological effects.
Pathways
SIRT6 plays a significant role in two key biological pathways: DNA damage response and metabolism regulation. In the DNA damage response pathway SIRT6 works with other proteins like PARP1 to facilitate DNA repair under stress conditions. In the regulation of metabolism SIRT6 interacts with transcription factors like HIF1α which influences the expression of genes involved in glycolytic metabolism and glucose homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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