Human SIVA1 (SIVA) knockout HEK-293T cell lysate
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SIVA1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 13 bp deletion in exon 1.
View Alternative Names
Apoptosis regulatory protein Siva, CD27 binding (Siva) protein, CD27-binding protein, CD27BP, SIVA_HUMAN, Siva 1, Siva 2
- Sanger seq
Unknown
Sanger Sequencing - Human SIVA1 (SIVA) knockout HEK-293T cell lysate (AB259114)
Homozygous : 13 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SIVA impacts apoptosis regulation through its interaction with signaling pathways that mediate cell death. It binds to the CD27 receptor a member of the tumor necrosis factor receptor (TNFR) family which is critical for the induction of apoptosis. SIVA is known to interact with other proteins such as those in the Bcl-2 family which further emphasize its role in orchestrating cell death programs.
Pathways
Various apoptotic cascades involve SIVA notably the extrinsic apoptotic signaling pathway linked to the TNFR family. This pathway engages the FasL/Fas and TNFR1 complexes where SIVA functions alongside other apoptosis regulators like caspases. SIVA also interfaces with proteins like TRAFs (tumor necrosis factor receptor-associated factors) which modulate signaling pathways related to immune responses and cell survival.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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