Human SLC12A9 knockout HeLa cell lysate
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SLC12A9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.
View Alternative Names
CCC-interacting protein 1, Cation-chloride cotransporter 6, Cation-chloride cotransporter-interacting protein 1, Potassium-chloride transporter 9, S12A9_HUMAN, SLC12A9, Solute carrier family 12 member 9, WO3.3, hCCC6, hCIP1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC12A9 knockout HeLa cell lysate (AB263348)
Homozygous : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein facilitates ion transport within cells contributing to osmoregulation and volume control. Although SLC12A9 is not a central part of any known large protein complex its ion transport functionality indicates it might interact with other membrane proteins involved in similar regulatory roles. This interaction helps modulate cell volume and osmolality which influences cell survival and function.
Pathways
SLC12A9 plays a role in the cellular signaling and homeostasis pathways. It is particularly associated with the WNK (With No Lysine) kinases-pathway which regulates electrolyte balance and blood pressure. In this context SLC12A9 has relations with other transporters like NKCC1 and KCC2 which also participate in the same ion transport and regulatory processes indicating cooperative interactions in maintaining cellular ionic balance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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