Human SLC22A5 (Solute carrier family 22 member 5) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SLC22A5 (Solute carrier family 22 member 5) knockout HeLa cell lysate (AB258672)
Allele-1 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC22A5 (Solute carrier family 22 member 5) knockout HeLa cell lysate (AB258672)
Allele-2 : 1 bp insertion in exon1
Complementary Products
Cell Lines & Lysates
AB265487
Human SLC22A5 (Solute carrier family 22 member 5) knockout HeLa cell line
- 0
- 0
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SLC22A5 functions to maintain carnitine homeostasis in the body. Carnitine is essential for the transport of long-chain fatty acids into the mitochondria for β-oxidation therefore providing cellular energy. SLC22A5 is a component of the carnitine transport system and interacts directly with other transporters and enzymes involved in the carnitine pathway. It is responsible for mediating the uptake and efflux of L-carnitine and acyl-carnitines across the cell membrane.
Pathways
The involvement of SLC22A5 in fatty acid metabolism is significant. It importantly participates in the carnitine shuttle allowing fatty acids to enter the mitochondria which is integral to energy production via fatty acid β-oxidation. SLC22A5 is linked with the long-chain fatty-acid-CoA ligase proteins which activate fatty acids prior to mitochondrial uptake. The interaction with these proteins ensures efficient energy production in response to cellular demands.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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