Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell lysate
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SLC25A10 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
SLC25A10, Solute carrier family 25 member 10
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell lysate (AB259118)
Allele-1 : 7 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell lysate (AB259118)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The mitochondrial dicarboxylate carrier aids in mitochondrial processes by mediating the shuttle of dicarboxylates important for the tricarboxylic acid (TCA) cycle and urea cycle. It actively participates in the transfer of metabolites that contribute to the synthesis of ATP linking important metabolic routes. The dicarboxylate carrier operates as a part of the family of mitochondrial transport proteins but does not form part of a larger protein complex.
Pathways
The dicarboxylate carrier intersects several key metabolic pathways including the TCA cycle and gluconeogenesis. Through these pathways the protein collaborates with others like the adenine nucleotide translocase; it aids in maintaining energetic homeostasis within cells. The correct functioning of the dicarboxylate carrier ensures the proper flow of carbon skeletons essential for cellular respiration and energy production.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266122
Human SLC25A10 (Mitochondrial dicarboxylate carrier) knockout HEK-293T cell line
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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