Human SLC25A13 (Citrin) knockout HeLa cell lysate
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SLC25A13 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3.
View Alternative Names
AI785475, ARALAR2, CMC2_HUMAN, CTLN2, Calcium-binding mitochondrial carrier protein Aralar2, Citrin, Ctrn, Mitochondrial aspartate glutamate carrier 2, RGD1565889, Slc25a13, Solute carrier family 25 (citrin) member 13, Solute carrier family 25 member 13, Solute carrier family 25 member 13 (citrin)
- WB
Lab
Western blot - Human SLC25A13 (Citrin) knockout HeLa cell lysate (AB258192)
Lane 1 : Wild-type HeLa cell lysate (20µg)
Lane 2 : SLC25A13 knockout HeLa cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control ab8245 observed at 37 kDa.
ab167166 Recombinant Anti-SLC25A13/Citrin antibody [EPR9969(B)] was shown to specifically react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (<a href='/en-us/products/primary-antibodies/slc25a13-citrin-antibody-epr9969b-ab167166'>ab167166</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SLC25A13 knockout HeLa cell lysate at 20 µg
Predicted band size: 74 kDa
Observed band size: 70 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A13 (Citrin) knockout HeLa cell lysate (AB258192)
Allele-1 : 1 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A13 (Citrin) knockout HeLa cell lysate (AB258192)
Allele-2 : 1 bp insertion in exon3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Citrin plays an important role in the urea cycle gluconeogenesis and lipid metabolism by facilitating the exchange of cytosolic glutamate for mitochondrial aspartate. It operates independently not as part of a larger protein complex. By ensuring the appropriate balance of amino acids and metabolites Citrin contributes to maintaining metabolic homeostasis. The protein's activity supports the liver's function and influences energy production efficiency especially under anabolic conditions.
Pathways
Citrin is integral to the malate-aspartate shuttle and the urea cycle. The malate-aspartate shuttle is involved in transferring reducing equivalents across the mitochondrial membrane which is essential for efficient ATP production. Citrin interacts closely with mitochondrial enzymes like carbamoyl phosphate synthetase I in the urea cycle emphasizing its link to ammonia detoxification. Citrin's transport activity indirectly supports the function of other mitochondrial carriers including SLC25A12 and SLC25A11 by maintaining the requisite balance of substrates required for their processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB167166
Anti-SLC25A13/Citrin antibody [EPR9969(B)]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (AB167166)](https://content.abcam.com/products/images/slc25a13-citrin-antibody-epr9969b-ab167166--western-blot-img1460.jpg)
- 0
- 0
Cell Lines & Lysates
AB265668
Human SLC25A13 (Citrin) knockout HeLa cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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