SLC25A13 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3.
AI785475, ARALAR2, CMC2_HUMAN, CTLN2, Calcium-binding mitochondrial carrier protein Aralar2, Citrin, Ctrn, Mitochondrial aspartate glutamate carrier 2, RGD1565889, Slc25a13, Solute carrier family 25 (citrin) member 13, Solute carrier family 25 member 13, Solute carrier family 25 member 13 (citrin)
SLC25A13 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3.
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Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
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The SLC25A13 protein also known as Citrin is a member of the solute carrier family 25 involved in transporting solutes across the mitochondrial membrane. Citrin functions as a calcium-dependent aspartate/glutamate carrier. It is around 67 kDa in mass. Citrin is primarily expressed in the liver heart and pancreas where it facilitates the exchange of metabolites necessary for cellular metabolism. The protein is localized to the inner mitochondrial membrane where it takes part in important cellular processes.
Citrin plays an important role in the urea cycle gluconeogenesis and lipid metabolism by facilitating the exchange of cytosolic glutamate for mitochondrial aspartate. It operates independently not as part of a larger protein complex. By ensuring the appropriate balance of amino acids and metabolites Citrin contributes to maintaining metabolic homeostasis. The protein's activity supports the liver's function and influences energy production efficiency especially under anabolic conditions.
Citrin is integral to the malate-aspartate shuttle and the urea cycle. The malate-aspartate shuttle is involved in transferring reducing equivalents across the mitochondrial membrane which is essential for efficient ATP production. Citrin interacts closely with mitochondrial enzymes like carbamoyl phosphate synthetase I in the urea cycle emphasizing its link to ammonia detoxification. Citrin's transport activity indirectly supports the function of other mitochondrial carriers including SLC25A12 and SLC25A11 by maintaining the requisite balance of substrates required for their processes.
Citrin deficiency is associated with two genetic conditions: Citrullinemia Type II and Neonatal Intrahepatic Cholestasis caused by Citrin Deficiency (NICCD). Both disorders arise from mutations in the SLC25A13 gene and disrupt normal urea cycle function leading to the accumulation of toxic substances like ammonia. Citrin's interaction with proteins involved in amino acid metabolism including SLC25A15 (ornithine transporter) highlights its role in maintaining nitrogen balance. Understanding Citrin's activity can aid in diagnosing and treating metabolic disorders linked to urea cycle dysfunction.
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Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: SLC25A13 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-SLC25A13/Citrin antibody [EPR9969(B)] ab167166 observed at 70 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-SLC25A13/Citrin antibody [EPR9969(B)] ab167166 Recombinant Anti-SLC25A13/Citrin antibody [EPR9969(B)] was shown to specifically react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human SLC25A13 (Citrin) knockout HeLa cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-SLC25A13/Citrin antibody [EPR9969(B)] ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (Anti-SLC25A13/Citrin antibody [EPR9969(B)] ab167166) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SLC25A13 knockout HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 74 kDa
Observed band size: 70 kDa
Allele-2: 1 bp insertion in exon3
Allele-1: 1 bp deletion in exon3
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