Human SLC25A22 (GC-1) knockout HEK-293T cell lysate
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SLC25A22 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.
View Alternative Names
GC-1, GHC1_HUMAN, Glutamate/H(+) symporter 1, Mitochondrial glutamate carrier 1, SLC25A22, Solute carrier family 25 member 22
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A22 (GC-1) knockout HEK-293T cell lysate (AB258673)
Allele-1 : 8 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A22 (GC-1) knockout HEK-293T cell lysate (AB258673)
Allele-2 : 1 bp insertion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GC-1 plays an important role in the signal transduction pathways involving nitric oxide as part of a complex with protein partners. As a subunit of the soluble guanylate cyclase (sGC) complex it helps mediate the effects of nitric oxide by regulating cGMP levels. This activity is essential for promoting vasodilation and blood flow which is observed in the cardiovascular system. The GC-1 complex translates extracellular signals to intracellular responses affecting various physiological processes.
Pathways
GC-1 is a critical component in the nitric oxide signaling pathway where it facilitates communication between nitric oxide and cGMP-dependent pathways. It interacts closely with proteins such as endothelial nitric oxide synthase (eNOS) and is directly involved in the pathway regulating vascular tone and blood pressure. Additionally GC-1 is part of the phototransduction pathway in retinal cells influencing the conversion of light into electrical signals through its production of cGMP.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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