Human SLC25A5 (Adenine Nucleotide Translocator 2/ANT 2) knockout HeLa cell lysate
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SLC25A5 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
2F1, AAC2, ADP, ADP ATP carrier protein 2, ADP/ATP translocase 2, ADT2_HUMAN, ANT 2, ATP carrier protein, ATP carrier protein 2, Adenine nucleotide translocator 2, OTTHUMP00000024324, SLC25A5, Solute carrier family 25 (mitochondrial carrier adenine nucleotide translocator) member 5, Solute carrier family 25 member 5, T2, T3, fibroblast isoform
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A5 (Adenine Nucleotide Translocator 2/ANT 2) knockout HeLa cell lysate (AB258676)
Allele-1 : 7 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC25A5 (Adenine Nucleotide Translocator 2/ANT 2) knockout HeLa cell lysate (AB258676)
Allele-2 : Insertion of the selection cassette in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ANT 2 functions to regulate cellular energy metabolism by controlling the exchange of adenine nucleotides between the mitochondria and cytosol. It is a part of the permeability transition pore complex which plays a role in maintaining mitochondrial membrane potential and preventing apoptosis. ANT 2 interacts with nucleotides to modulate energy production making it essential for the maintenance of cellular bioenergetics.
Pathways
ANT 2 integrates into significant energy production pathways including oxidative phosphorylation. It forms a functional unit with proteins like the voltage-dependent anion channel (VDAC) within the mitochondrial interface. This relationship with VDAC is key to ATP production and involves cellular energy transactions fundamental to numerous metabolic and signaling pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB265314
Human SLC25A5 (Adenine Nucleotide Translocator 2/ANT 2) knockout HeLa cell line
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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