Human SLC30A1 (ZnT1) knockout HeLa cell lysate
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SLC30A1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon 1 and 3 bp deletion in exon 1.
View Alternative Names
SLC30 A1, SLC30A 1, SLC30A1, Solute carrier family 30 (zinc transporter), member 1, Solute carrier family 30 member 1, ZNT1_HUMAN, ZRC 1, Zinc transporter 1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC30A1 (ZnT1) knockout HeLa cell lysate (AB258678)
Allele-1 : 20 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC30A1 (ZnT1) knockout HeLa cell lysate (AB258678)
Allele-2 : 3 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ZnT1 is essential for regulating the cellular zinc levels and protecting against zinc toxicity. It does not form part of a larger complex; instead it operates as an individual unit to ensure that excess zinc does not accumulate to harmful levels within the cell. By controlling zinc efflux ZnT1 influences processes such as cell signaling and apoptosis which rely on zinc as a cofactor or structural component.
Pathways
Homeostasis of zinc ions in cells connects ZnT1 to the cellular zinc transport pathway and the metal ion transport pathway. ZnT1 coordinates with intracellular zinc-binding proteins like metallothioneins to modulate zinc availability and transportation within the cell. Proteins such as ZIP4 which facilitate zinc uptake operate in a complementary manner to ZnT1 balancing zinc influx and efflux to maintain cellular function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB258523
Human MPI (Mannose Phosphate Isomerase) knockout HEK-293T cell lysate
- 0
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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