Human SLC30A7 (ZnT-7) knockout HeLa cell lysate
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SLC30A7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1.
View Alternative Names
1810059J10Rik, 2610034N15Rik, 4833428C12Rik, AI467242, DKFZp686M0368, SLC30A7, Solute carrier family 30 (zinc transporter), member 7, Solute carrier family 30 member 7, ZNT7_HUMAN, ZNTL2, Zinc transporter 7, Znt-like transporter 2
- Sanger seq
Unknown
Sanger Sequencing - Human SLC30A7 (ZnT-7) knockout HeLa cell lysate (AB263352)
Allele-1 : 4 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC30A7 (ZnT-7) knockout HeLa cell lysate (AB263352)
Allele-2 : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ZnT-7 participates in the regulation of cellular zinc balance and is important for maintaining optimal enzyme activity and protein function. Although it does not form part of a larger complex itself it interacts with other zinc transporters to help coordinate zinc distribution within cells. This management of zinc levels is important for preventing zinc toxicity and supporting normal cellular activities which are dependent on zinc as a cofactor.
Pathways
ZnT-7 is involved in the zinc homeostasis pathway controlling the intracellular sequestration and distribution of zinc. It works alongside other zinc transporters such as ZnT-1 and ZIP family members to maintain appropriate intracellular zinc levels. ZnT-7 also affects the insulin signaling pathway as zinc ions are modulators of insulin storage and secretion in pancreatic beta cells.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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