Human SLC38A9 knockout HeLa cell lysate
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SLC38A9 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 5 bp deletion in exon3.
View Alternative Names
FLJ46104, FLJ90709, Homo sapiens solute carrier family 38, member 9, MGC120544, OTTHUMP00000161835, Putative sodium-coupled neutral amino acid transporter 9, Putative uncharacterized protein FLJ90709, S38A9_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human SLC38A9 knockout HeLa cell lysate (AB258681)
Allele-2 : 1 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC38A9 knockout HeLa cell lysate (AB258681)
Allele-1 : 5 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein serves as a component of the mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway. SLC38A9 directly interacts with the lysosomal surface to connect amino acid availability to mTORC1 activation. By doing so it influences cell growth and autophagy processes in response to amino acid levels. As a part of the mTORC1 complex SLC38A9 plays an important function in translating extracellular cues to intracellular responses.
Pathways
SLC38A9 is integral in the amino acid sensing mechanism that regulates the mTORC1 pathway. This pathway controls vital processes like protein synthesis and cell proliferation. The protein partners with other key players in the mTOR signaling pathway including Rag GTPases and Raptor to relay amino acid abundance to the mTORC1 complex. This relationship ensures that cellular growth processes are synchronized with available nutrition resources.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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