Human SLC39A1 knockout HeLa cell lysate
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SLC39A1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and Insertion of the selection cassette in exon 3.
View Alternative Names
IRT 1, S39A1_HUMAN, Solute carrier family 39 member 1, ZIP-1, ZIRTL, Zinc transporter ZIP1, Zinc-iron-regulated transporter-like, Zrt- and Irt-like protein 1, hZIP1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC39A1 knockout HeLa cell lysate (AB259132)
Allele-2 : Insertion of the selection cassette in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC39A1 knockout HeLa cell lysate (AB259132)
Allele-1 : 1 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SLC39A1 protein plays an essential role in cellular zinc uptake by increasing cytoplasmic zinc concentrations. This protein is not part of a larger complex but interacts with zinc ions directly ensuring adequate zinc availability for numerous cellular processes. Zinc is a critical cofactor for many enzymes and is necessary for proper immune function protein synthesis and cellular proliferation. By regulating zinc levels in cells SLC39A1 supports diverse physiological functions and maintains cellular health.
Pathways
SLC39A1 participates in zinc homeostasis and signal transduction pathways. It is pivotal in the regulation of the metallothionein expression pathway which helps detoxify heavy metals and protect against oxidative stress. Also SLC39A1 interacts with the NF-κB signaling pathway where zinc acts as a second messenger influencing cell survival and immune response. Proteins such as MT (metallothionein) and IκBα interact with SLC39A1 in these pathways thereby linking zinc transport to broader cellular functions and responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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