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AB257680

Human SLC3A2 (CD98) knockout HeLa cell lysate

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SLC3A2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon6.

View Alternative Names

4F2 cell-surface antigen heavy chain, 4F2 heavy chain, 4F2 heavy chain antigen, 4F2_HUMAN, 4F2hc, 4T2HC, Antigen defined by monoclonal antibody 4F2 heavy chain, Antigen identified by monoclonal antibodies 4F2 TRA1.10 TROP4 and T43, CD98 antigen, CD98 heavy chain, CD98HC, Lymphocyte activation antigen 4F2 large subunit, MDU 1, Monoclonal antibody 44D7, NACAE, Slc3a2, Solute carrier family 3 (activators of dibasic and neutral amino acid transport) member 2, Solute carrier family 3 member 2

2 Images
Western blot - Human SLC3A2 (CD98) knockout HeLa cell lysate (AB257680)
  • WB

Lab

Western blot - Human SLC3A2 (CD98) knockout HeLa cell lysate (AB257680)

Lane 1 : Wild-type HeLa cell lysate 20 μg

Lane 2 : SLC3A2 knockout HeLa cell lysate 20 μg

Lane 3 : MCF7 cell lysate 20 μg

Lane 4 : Jurkat cell lysate 20 μg

Lane 5 : A431 cell lysate 20 μg

Lanes 1 - 5 : Merged signal (red and green). Green - ab253273 observed at 80 - 90 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

ab253273 was shown to react with CD98 in wild-type HeLa cells in Western blot with loss of signal observed in SLC3A2 knockout cell line ab265708 (SLC3A2 knockout cell lysate ab257680). Wild-type HeLa and SLC3A2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5 % milk in TBS-T (0.1 % Tween®) before incubation with ab253273 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Human SLC3A2 (CD98) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-slc3a2-cd98-knockout-hela-cell-line-ab265708'>ab265708</a>)

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Sanger Sequencing - Human SLC3A2 (CD98) knockout HeLa cell lysate (AB257680)
  • Sanger seq

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Sanger Sequencing - Human SLC3A2 (CD98) knockout HeLa cell lysate (AB257680)

Homozygous : 14 bp deletion in exon6

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon6.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Sanger seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
SLC3A2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD98 also known as CD98hc CD98 protein or LAT1/CD98 is a type II membrane protein often found in association with the heavy chains of amino acid transporters like LAT1. It has a molecular mass of approximately 85-100 kDa. CD98 is widely expressed in numerous tissues including the liver kidney and various tumors. It plays an important role in cellular processes by facilitating the transport of amino acids across plasma membranes which is essential for maintaining cellular homeostasis and proliferation.
Biological function summary

CD98 forms a complex with light chains of the heterodimeric amino acid transporters such as LAT1 and LAT2. This interaction enhances the uptake of neutral amino acids into cells. CD98 also interacts with integrins influencing cell adhesion and migration which are critical for cellular signaling and response. Through these interactions CD98 impacts not only cell survival and growth but also immune responses and angiogenesis.

Pathways

CD98 is a vital component of the mTOR signaling pathway influencing cell growth and proliferation. It also plays a part in the integrin signaling pathway which regulates cell adhesion and migration. In these pathways CD98 works closely with proteins like FG1 and mTORC1. These relationships enable it to modulate cellular responses to nutritional availability and mechanical cues from the environment.

CD98 expression and function have links to cancer and autoimmune diseases. High expression levels of CD98 are observed in various cancers such as lymphomas where it correlates with increased tumor growth and poor prognosis. In autoimmune disorders like rheumatoid arthritis CD98 influences immune cell activation and migration. Proteins such as CP1 and FG1 interact with CD98 contributing to disease pathology by affecting cellular and immune responses.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Cell Lines & Lysates

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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