Human SLC41A3 knockout HEK-293T cell lysate
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SLC41A3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
View Alternative Names
1010001P06Rik, AI480742, FLJ20473, OTTMUSP00000028993, OTTMUSP00000028994, SLC41A1 L2, SLC41A1 like 2, Solute carrier family 41, member 3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC41A3 knockout HEK-293T cell lysate (AB259134)
Homozygous : 1 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SLC41A3 participates in maintaining the cellular magnesium balance essential for many cellular processes such as enzyme activation and ion channel regulation. The protein acts individually; however it may interact with other magnesium transport proteins to achieve this balance. The maintenance of magnesium levels is necessary for proper cellular function and biochemical reactions.
Pathways
The SLC41A3 protein integrates into the magnesium homeostasis pathway which involves other proteins such as TRPM7 and CNNM2. Within these pathways SLC41A3 contributes to essential physiological processes including muscle contraction and neural activity. Its function is implicit in transport and regulation mechanisms that keep magnesium concentrations within healthy ranges.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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