Human SLC43A3 knockout HeLa cell lysate
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SLC43A3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3.
View Alternative Names
DKFZp762A227, EEG1, FOAP 13, PRO1659, SEEEG 1, likely ortholog of mouse embryonic epithelial gene 1, solute carrier family 43 member 3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC43A3 knockout HeLa cell lysate (AB259135)
Homozygous : 1 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein ensures the uptake of essential nucleobases contributing to nucleotide biosynthesis and salvage pathways. SLC43A3 does not function as part of a larger protein complex. Its role in transporting nucleobases is significant for cellular RNA and DNA synthesis indicating its involvement in cellular proliferation and repair. This highlights its importance in cellular growth and energy metabolism.
Pathways
SLC43A3 actively contributes to the purine salvage pathway critical for recycling nucleobases back into nucleotides. The purine salvage pathway is integral for conserving energy in cells as it allows the reuse of preformed bases. SLC43A3 is closely related to proteins like HPRT1 and APRT which also participate in this pathway. These interactions enable the efficient functioning of nucleotide metabolism and synthesis within various cell types.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
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