Human SLC50A1 (RAG1AP1) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SLC50A1 (RAG1AP1) knockout HeLa cell lysate (AB259137)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC50A1 (RAG1AP1) knockout HeLa cell lysate (AB259137)
Allele-3 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SLC50A1 (RAG1AP1) knockout HeLa cell lysate (AB259137)
Allele-1 : 2 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The activity of RAG1AP1 involves modulating the immune system through its role in gene regulation. It participates in a complex interaction with proteins involved in the DNA damage response which is important for maintaining genomic stability. RAG1AP1 impacts the activation of genes necessary for immunological processes influencing how cells respond to immune challenges.
Pathways
RAG1AP1 plays a role in adaptive immunity and DNA repair pathways. The protein integrates into the broader context of the V(D)J recombination pathway directly interacting with proteins like RAG1 and RAG2 that are involved in recombination events essential for antibody diversity. Additionally RAG1AP1 relates to ubiquitination pathways that affect protein degradation and stability including factors like DCAF1.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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