Human SLC52A2 (GPR172A/PAR1) knockout HeLa cell lysate
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SLC52A2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
GPR172A, PERV-A receptor 1, Porcine endogenous retrovirus A receptor 1, Protein GPR172A, RFT3, RFT3_HUMAN, Riboflavin transporter 3, hRFT3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC52A2 (GPR172A/PAR1) knockout HeLa cell lysate (AB263357)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SLC52A2 (GPR172A/PAR1) knockout HeLa cell lysate (AB263357)
Allele-1 : 1 bp deletion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GPR172A/PAR1 plays an important role in regulating blood clotting and inflammation. It is part of a signaling complex that includes thrombin a serine protease. This complex is important for activating platelets which are cells involved in the blood clotting process. By activating platelets GPR172A/PAR1 helps control bleeding and wound healing.
Pathways
The protein is involved in both the coagulation and inflammatory pathways. In the coagulation pathway it works alongside other proteins like thrombin and fibrinogen contributing to clot formation. In inflammation GPR172A/PAR1 interacts with proteins such as NF-kB helping to regulate inflammatory responses and cytokine production which are important for immune defense and tissue repair.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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