Human SLC5A6 knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SLC5A6 knockout HEK-293T cell lysate (AB259139)
Allele-1 : 7 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC5A6 knockout HEK-293T cell lysate (AB259139)
Allele-2 : 1 bp deletion in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SLC5A6 is essential for the efficient transport of vitamins across cell membranes using sodium gradient as the driving force. It transports vitamins into cells where they participate as coenzymes in several enzymatic reactions. This transporter does not form part of a larger protein complex but functions individually to link dietary intake with cellular nutrient requirements. The absorption through SLC5A6 impacts various biosynthetic processes as biotin and pantothenate contribute to fatty acid synthesis and energy metabolism.
Pathways
The role of SLC5A6 in nutrient absorption connects it to critical metabolic pathways such as the biotin and CoA biosynthetic pathways. Its function impacts cellular metabolism by providing essential cofactors for carboxylation reactions and energy production. SLC5A6 interacts with enzymes that rely on these vitamin-derived cofactors maintaining cellular metabolic equilibrium essential for cell growth and energy balance.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com