Human SLC7A1 (CAT1) knockout HEK-293T cell lysate
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SLC7A1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 35 bp deletion in exon3.
View Alternative Names
ATRC1, Amino acid transporter cationic 1, CTR1_HUMAN, ERR, Ecotropic retroviral leukemia receptor homolog, Ecotropic retroviral receptor, Ecotropic retrovirus receptor homolog, HCAT1, High-affinity cationic amino acid transporter-1, REC1L, Slc7a1, Solute carrier family 7 (cationic amino acid transporter, y+ system) member 1, Solute carrier family 7 member 1, System Y+ basic amino acid transporter
- Sanger seq
Unknown
Sanger Sequencing - Human SLC7A1 (CAT1) knockout HEK-293T cell lysate (AB257682)
Allele-2 : 1 bp insertion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human SLC7A1 (CAT1) knockout HEK-293T cell lysate (AB257682)
Allele-1 : 35 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CAT1 influences nitric oxide production by regulating arginine availability a substrate for nitric oxide synthase. By controlling amino acid imports it affects cellular signaling and metabolism. CAT1 does not function as part of a larger complex but interacts with other transporters to maintain cellular amino acid balance. CAT1's role extends to impacting cell proliferation as cationic amino acids are important for growing tissues.
Pathways
CAT1 has been implicated in the nitric oxide synthesis pathway where it contributes by sustaining intracellular arginine levels. This involvement is critical to vascular function and immune response regulation. CAT1 also participates in mTOR signaling by providing necessary amino acids like arginine which influence protein synthesis and cellular growth. In these pathways CAT1 connects with proteins such as mTOR and nitric oxide synthase assisting in integral cellular functions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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