Human SLC7A6 knockout HeLa cell lysate
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SLC7A6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and Insertion of the selection cassette in exon 4.
View Alternative Names
AI643885, Cationic amino acid transporter y+ system, DKFZp686K15246, KIAA0245, Solute carrier family 7 (cationic amino acid transporter y+ system) member 6, Solute carrier family 7 member 6, Y+L amino acid transporter 2, y(+)L type amino acid transporter 2, y+LAT 2
- Sanger seq
Unknown
Sanger Sequencing - Human SLC7A6 knockout HeLa cell lysate (AB259140)
Allele-1 : 1 bp deletion in exon 4
- Sanger seq
Unknown
Sanger Sequencing - Human SLC7A6 knockout HeLa cell lysate (AB259140)
Allele-2 : Insertion of the selection cassette in exon 4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The SLC7A6 protein participates in amino acid transport across the plasma membrane and it forms a functional complex with the glycoprotein 4F2hc/CD98. This association is necessary for the optimal activity and localization of SLC7A6 on the cell surface. Its role in amino acid exchange impacts nutrient sensing and plays a critical function in cellular growth and repair processes. The protein is involved in maintaining amino acid balance which is fundamental in many physiological processes.
Pathways
Amino acid transporters like SLC7A6 are significant in metabolic and mTOR signaling pathways. These pathways influence cellular growth proliferation and metabolism. SLC7A6 is closely related to other transporters like SLC7A5 within these pathways catalyzing the bidirectional movement of large neutral amino acids. It ensures the availability of essential amino acids for protein synthesis and energy production affecting the overall metabolic state of the cell.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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