Human SLC9A3R1 (EBP50/NHERF-1) knockout HCT116 cell lysate
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NHERF1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon1.
View Alternative Names
EBP 50, Ezrin-radixin-moesin-binding phosphoprotein 50, NHERF, NHERF-1, NHRF1_HUMAN, NPHLOP2, Na(+)/H(+) exchange regulatory cofactor NHE RF, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co factor, Regulatory cofactor of Na(+)/H(+) exchanger, Slc9a3r1, Sodium-hydrogen exchanger regulatory factor 1, Sodium/hydrogen exchanger regulatory factor 1, Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 1, Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1, Solute carrier family 9 isoform 3 regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1, Solute carrier family 9 member 3 regulator 1
- WB
Lab
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HCT116 cell lysate (AB257281)
Lane 1 : Wild-type HCT116 cell lysate (20µg)
Lane 2 : SLC9A3R1 knockout HCT116 cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab109430 observed at 48 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109430 Anti-EBP50/NHERF-1 antibody [EPR5562] was shown to specifically react with EBP50/NHERF-1 in wild-type HCT116 cells in western blot. Loss of signal was observed when knockout cell line ab266876 (knockout cell lysate ab257281) was used. Wild-type and EBP50/NHERF-1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109430 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-EBP50/NHERF-1 antibody [EPR5562] (<a href='/en-us/products/primary-antibodies/ebp50-nherf-1-antibody-epr5562-ab109430'>ab109430</a>) at 1/1000 dilution
Lane 1:
Wild-type HCT116 cell lysate at 20 µg
Lane 2:
SLC9A3R1 knockout HCT116 cell lysate at 20 µg
Lane 2:
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-slc9a3r1-ebp50-nherf-1-knockout-hct116-cell-line-ab266876'>ab266876</a>)
Predicted band size: 38 kDa
Observed band size: 48 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SLC9A3R1 (EBP50/NHERF-1) knockout HCT116 cell lysate (AB257281)
Homozygous : 1 bp deletion in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EBP50 regulates cellular functions by organizing signaling complexes at the plasma membrane. It is involved in processes such as ion transport signal transduction and cell proliferation. EBP50 often acts as part of a multiprotein complex contributing to the stabilization and regulation of membrane receptors and channels. By regulating these components EBP50 affects a variety of cellular responses to external stimuli.
Pathways
EBP50 plays important roles in cellular signaling pathways like the PTH (parathyroid hormone) receptor signaling and CFTR (cystic fibrosis transmembrane conductance regulator) channel regulation. It interacts with proteins such as ezrin and PLCβ (phospholipase C beta) which facilitates the signaling cascades critical for maintaining proper cell function. By modulating these pathways EBP50 has a significant impact on maintaining cellular homeostasis and responding to changing physiological conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Cell Lines & Lysates
AB266876
Human SLC9A3R1 (EBP50/NHERF-1) knockout HCT116 cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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