Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell lysate
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- WB
Lab
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell lysate (AB257280)
False colour image of Western blot : Anti-EBP50/NHERF-1 antibody [EPR5562] staining at 1/1000 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab109430 was shown to bind specifically to EBP50/NHERF-1. A band was observed at 46 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLC9A3R1 knockout cell line ab264914 (knockout cell lysate ab257280). To generate this image wild-type and SLC9A3R1 knockout HeLa cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-EBP50/NHERF-1 antibody [EPR5562] (<a href='/en-us/products/primary-antibodies/ebp50-nherf-1-antibody-epr5562-ab109430'>ab109430</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SLC9A3R1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-slc9a3r1-ebp50-nherf-1-knockout-hela-cell-line-ab264914'>ab264914</a>)
Lane 2:
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell lysate (ab257280)
Predicted band size: 38 kDa
Observed band size: 46 kDa
false
- WB
Lab
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell lysate (AB257280)
False colour image of Western blot : Anti-EBP50/NHERF-1 antibody staining at 1 μg/ml shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab88238 was shown to bind specifically to EBP50/NHERF-1. A band was observed at 46 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLC9A3R1 knockout cell line ab264914 (knockout cell lysate ab257280). To generate this image wild-type and SLC9A3R1 knockout HeLa cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-EBP50/NHERF-1 antibody (<a href='/en-us/products/primary-antibodies/ebp50-nherf-1-antibody-ab88238'>ab88238</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SLC9A3R1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-slc9a3r1-ebp50-nherf-1-knockout-hela-cell-line-ab264914'>ab264914</a>)
Lane 2:
Western blot - Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell lysate (ab257280)
Predicted band size: 38 kDa
Observed band size: 46 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SLC9A3R1 (EBP50/NHERF-1) knockout HeLa cell lysate (AB257280)
Homozygous : 19 bp deletion in exon 1
Complementary Products
Cell Lines & Lysates
AB266876
Human SLC9A3R1 (EBP50/NHERF-1) knockout HCT116 cell line
Advanced Validation
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
EBP50 regulates cellular functions by organizing signaling complexes at the plasma membrane. It is involved in processes such as ion transport signal transduction and cell proliferation. EBP50 often acts as part of a multiprotein complex contributing to the stabilization and regulation of membrane receptors and channels. By regulating these components EBP50 affects a variety of cellular responses to external stimuli.
Pathways
EBP50 plays important roles in cellular signaling pathways like the PTH (parathyroid hormone) receptor signaling and CFTR (cystic fibrosis transmembrane conductance regulator) channel regulation. It interacts with proteins such as ezrin and PLCβ (phospholipase C beta) which facilitates the signaling cascades critical for maintaining proper cell function. By modulating these pathways EBP50 has a significant impact on maintaining cellular homeostasis and responding to changing physiological conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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