SLC9A3R2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 3.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 3.
Alternative names
E3KARP, HGNC:11076, Isoform 3 regulator 2, Isoform 3 regulatory factor 2, MGC104639, NHE3 kinase A regulatory protein, NHE3 kinase A regulatory protein E3KARP, NHE3 regulatory factor 2, NHE3RF2, NHERF-2, NHRF2_HUMAN, Na(+)/H(+) exchange regulatory cofactor NHE-RF2, OCTS2, SIP-1, SRY-interacting protein 1, Slc9a3r2, Sodium-hydrogen exchanger regulatory factor 2, Sodium/hydrogen exchanger, Sodium/hydrogen exchanger regulatory factor 2, Solute carrier family 9 (sodium/hydrogen exchanger), Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulator 2, Solute carrier family 9 (sodium/hydrogen exchanger) isoform 3 regulatory factor 2, Solute carrier family 9 (sodium/hydrogen exchanger) member 3 regulator 2, Solute carrier family 9 isoform 3 regulator 2, Solute carrier family 9 isoform A3 regulatory factor 2, TKA-1, Tyrosine kinase activator protein 1
What's included?
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SLC9A3R2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 3.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 3.
- Concentration
Properties
- Gene name
- SLC9A3R2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Human SLC9A3R2 (NHERF-2/SIP-1) knockout HEK-293T cell lysate (ab258685)
Lane 1:Wild-type HEK293T cell lysate (20 ug)
Lane 2:SLC9A3R2 knockout HEK293T cell lysate (20 ug)
Lane 3:MCF7 cell lysate (20 ug)
Lane 4:T-47D cell lysate (20 ug)Anti-NHERF-2/SIP-1 antibody [32B6] ab151443 was shown to specifically react with NHERF-2/SIP-1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human SLC9A3R2 (NHERF-2/SIP-1) knockout HEK-293T cell line ab266778 (knockout cell lysate ab258685) was used. Wild-type and NHERF-2/SIP-1 knockout samples were subjected to SDS-PAGE. Anti-NHERF-2/SIP-1 antibody [32B6] ab151443 and Anti-beta Tubulin [EP1331Y] - Microtubule Marker (Anti-beta Tubulin antibody [EP1331Y] - Microtubule Marker ab52901) were incubated overnight at 4oC at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Sanger Sequencing - Human SLC9A3R2 (NHERF-2/SIP-1) knockout HEK-293T cell lysate (ab258685)
Homozygous: 11 bp deletion in exon 3
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