Human SLCO4A1 knockout HeLa cell lysate
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SLCO4A1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
Colon organic anion transporter, OATP-E, OATP-RP1, OATP1, OATP4A1, Organic anion transporter polypeptide-related protein 1, Organic anion-transporting polypeptide E, POAT, SLC21A12, SO4A1_HUMAN, Sodium-independent organic anion transporter E, Solute carrier family 21 (organic anion transporter) member 12, Solute carrier family 21 member 12, Solute carrier organic anion transporter family member 4A1
- Sanger seq
Unknown
Sanger Sequencing - Human SLCO4A1 knockout HeLa cell lysate (AB259141)
Allele-3 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human SLCO4A1 knockout HeLa cell lysate (AB259141)
Allele-2 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human SLCO4A1 knockout HeLa cell lysate (AB259141)
Allele-1 : 1 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SLCO4A1 is engaged in the transport of hormones drugs and metabolites across cellular compartments. It belongs to the larger family of solute carriers and does not necessarily form part of a multi-protein complex operating as a standalone transporter. Its activity influences the cellular internalization of various biologically active compounds marking its importance in balancing intracellular and extracellular concentrations.
Pathways
SLCO4A1 integration is seen in critical pathways like drug metabolism and steroid hormone metabolism. In the context of drug metabolism it works alongside cytochrome P450 enzymes to ensure the proper processing and elimination of pharmaceutical compounds. In steroid hormone metabolism it interacts with proteins such as SHBG (sex hormone-binding globulin) to modulate hormone bioavailability influencing physiological responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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