Human SLPI knockout HeLa cell lysate
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SLPI KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
View Alternative Names
ALP, WAP4, WAP four-disulfide core domain protein 4, Mucus proteinase inhibitor, Antileukoproteinase, BLPI, HUSI-1, Secretory leukocyte protease inhibitor, Protease inhibitor WAP4, MPI, Seminal proteinase inhibitor, SLPI, WFDC4
- WB
Lab
Western blot - Human SLPI knockout HeLa cell lysate (AB257685)
False colour image of Western blot : Anti-SLPI antibody - Aminoterminal end staining at 1/1000 dilution shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution shown in red. In Western blot ab46763 was shown to bind specifically to SLPI. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLPI knockout cell line ab265215 (knockout cell lysate ab257685). To generate this image wild-type and SLPI knockout HeLa cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye®800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-SLPI antibody - Aminoterminal end (<a href='/en-us/products/primary-antibodies/slpi-antibody-aminoterminal-end-ab46763'>ab46763</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SLPI knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human SLPI knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-slpi-knockout-hela-cell-line-ab265215'>ab265215</a>)
Lane 2:
Western blot - Human SLPI knockout HeLa cell lysate (ab257685)
Lane 3:
PC-3 cell lysate at 20 µg
Lane 4:
LNCaP cell lysate at 20 µg
Predicted band size: 14 kDa
Observed band size: 22 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human SLPI knockout HeLa cell lysate (AB257685)
Homozygous : Insertion of the selection cassette in exon1
Complementary Products
Primary Antibodies
AB243019
Anti-SLPI antibody [EPR21765-42] - BSA and Azide free (Capture)
RabMAb|Recombinant
![Sandwich ELISA - Anti-SLPI antibody [EPR21765-42] - BSA and Azide free (Capture) (AB243019)](https://content.abcam.com/products/images/slpi-antibody-epr21765-42-bsa-and-azide-free-capture-ab243019--sandwich-elisa-img49996.jpg)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SLPI serves as a protector of epithelial surfaces from proteolytic degradation and acts as an anti-inflammatory agent. It displays broad-spectrum antimicrobial properties providing protection against bacterial and viral infections. Although SLPI is not part of a complex itself its interactions with extracellular matrix proteins help modulate wound healing processes and maintain tissue integrity.
Pathways
SLPI participates in the innate immune response and inflammation regulation pathways. It interacts within pathways that involve the complement cascade and kallikrein-kinin system helping to regulate inflammation and modulate immune responses. During these processes SLPI is related to proteins such as alpha-1-antitrypsin in its role as a protease inhibitor contributing to the regulation of protease activity and inflammatory responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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