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AB257686

Human SMAD1 knockout HeLa cell lysate

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SMAD1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3.
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Sanger Sequencing - Human SMAD1 knockout HeLa cell lysate (AB257686)
  • Sanger seq

Unknown

Sanger Sequencing - Human SMAD1 knockout HeLa cell lysate (AB257686)

Allele-2 : 1 bp insertion in exon3

Sanger Sequencing - Human SMAD1 knockout HeLa cell lysate (AB257686)
  • Sanger seq

Unknown

Sanger Sequencing - Human SMAD1 knockout HeLa cell lysate (AB257686)

Allele-1 : 1 bp deletion in exon3

Western blot - Human SMAD1 knockout HeLa cell lysate (AB257686)
  • WB

Lab

Western blot - Human SMAD1 knockout HeLa cell lysate (AB257686)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : SMAD1 knockout HeLa cell lysate (20µg)

Lane 3 : HT1080 cell lysate (20µg)

Lane 4 : Huvec cell lysate (20µg)

Lanes 1- 4 : Merged signal (red and green). Green - ab126761 observed at 52 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab126761 Anti-Smad1 antibody [EPR5522] was shown to specifically react with Smad1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265400 (knockout cell lysate ab257686) was used. Wild-type and Smad1 knockout samples were subjected to SDS-PAGE. ab126761 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Smad1 antibody [EPR5522] (<a href='/en-us/products/primary-antibodies/smad1-antibody-epr5522-ab126761'>ab126761</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SMAD1 knockout HeLa cell lysate (ab257686) at 20 µg

Lane 3:

HT1080 cell lysate at 20 µg

Lane 4:

Huvec cell lysate at 20 µg

Predicted band size: 52 kDa

Observed band size: 52 kDa

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Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB33902

Anti-Smad1 antibody [EP565Y]

20ul selling size|KO Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Smad1 antibody [EP565Y] (AB33902)

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Proteins & Peptides

AB84653

Recombinant Human Smad1 protein

Western blot - Recombinant Human Smad1 protein (AB84653)

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Assay Kits

AB186036

SMAD1 (pS463/465) ELISA Kit

SimpleStep

ELISA - SMAD1 (pS463/465) ELISA Kit (AB186036)

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Cell Lines & Lysates

AB258036

Human LUC7L2 knockout HEK-293T cell lysate

Sanger Sequencing - Human LUC7L2 knockout HEK-293T cell lysate (AB258036)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
SMAD1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad1 also known as Mothers against decapentaplegic homolog 1 (MADH1) is a protein encoded by the SMAD1 gene in humans. It has a molecular mass of approximately 53 kDa and functions as a receptor-regulated Smad protein. Smad1 primarily transmits signals from the bone morphogenetic proteins (BMPs) a group of growth factors and cytokines. Expression of Smad1 occurs in various tissues including the lung heart and kidney. It plays an important role in mediating signaling pathways within different cellular environments.
Biological function summary

Smad1 participates in the transmission of BMP signals from the cell surface to the nucleus ensuring transcriptional regulation of target genes. In the presence of BMPs Smad1 forms complexes with Smad4 upon activation. These Smad complexes then translocate to the nucleus where they regulate gene expression. Smad1 influences cellular responses such as proliferation differentiation and apoptosis. In particular it plays a significant role in bone development and osteogenesis.

Pathways

Smad1 plays an important role within the BMP signaling pathway which is important for early development and tissue homeostasis. Within this pathway BMPs trigger the phosphorylation of Smad1 which then associates with Smad4 to propagate downstream signaling. Another associated pathway includes the TGF-beta signaling pathway where Smads like Smad2 and Smad3 show functional similarities and differences with Smad1. The interactions of Smad1 with related proteins like Smad4 highlight its significant contribution to cellular processes regulated by these pathways.

Smad1 associates with various pathological conditions. For instance aberrant Smad1 signaling has implications in cancer particularly in processes like tumor progression and metastasis. Disruptions in the BMP pathway involving Smad1 and Smad4 can influence the onset and development of disorders such as pulmonary hypertension where inappropriate cell growth and remodeling occur. Understanding Smad1's role in these contexts provides insights into potential therapeutic avenues for treatment and management of these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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