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AB258198

Human SMARCC1 (BAF155) knockout HeLa cell lysate

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SMARCC1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon 2 and 9 bp deletion in exon 2.

View Alternative Names

AI115498, BAF 155, BRG1-associated factor 155, CRACC 1, Chromatin remodeling complex BAF155 subunit, Mammalian chromatin remodeling complex BRG1 associated factor 155, Rsc 8, SMARC C1, SMRC1_HUMAN, SRG 3, SWI 3, SWI/SNF complex 155 kDa subunit, SWI/SNF complex subunit SMARCC1, SWI/SNF related matrix associated actin dependent regulator of chromatin c1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1

3 Images
Western blot - Human SMARCC1 (BAF155) knockout HeLa cell lysate (AB258198)
  • WB

Lab

Western blot - Human SMARCC1 (BAF155) knockout HeLa cell lysate (AB258198)

Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SMARCC1 knockout HeLa cell lysate 20 μg
Lanes 1 - 2 : Merged signal (red and green). Green - ab172638 observed at 112 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab172638 was shown to react with SMARCC1 in wild-type HeLa cells in Western blot with loss of signal observed in SMARCC1 knockout cell line ab264859 (SMARCC1 knockout cell lysate ab258198). Wild-type HeLa and SMARCC1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab172638 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-SMARCC1/BAF155 antibody [EPR12395] - ChIP Grade (<a href='/en-us/products/primary-antibodies/smarcc1-baf155-antibody-epr12395-chip-grade-ab172638'>ab172638</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SMARCC1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SMARCC1 (BAF155) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-smarcc1-baf155-knockout-hela-cell-line-ab264859'>ab264859</a>)

Predicted band size: 123 kDa

Observed band size: 112 kDa

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Sanger Sequencing - Human SMARCC1 (BAF155) knockout HeLa cell lysate (AB258198)
  • Sanger seq

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Sanger Sequencing - Human SMARCC1 (BAF155) knockout HeLa cell lysate (AB258198)

Allele-1 : 9 bp deletion in exon 2

Sanger Sequencing - Human SMARCC1 (BAF155) knockout HeLa cell lysate (AB258198)
  • Sanger seq

Unknown

Sanger Sequencing - Human SMARCC1 (BAF155) knockout HeLa cell lysate (AB258198)

Allele-2 : 8 bp deletion in exon 2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon 2 and 9 bp deletion in exon 2.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
SMARCC1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMARCC1 also known as BAF155 is a component of the SWI/SNF chromatin remodeling complex with a molecular weight of approximately 155 kDa. It plays a role in modifying the structure of chromatin which affects the accessibility of DNA to transcription factors and other proteins. SMARCC1 expresses widely in various tissues particularly where regulation of gene transcription is essential. This protein acts as a scaffold facilitating the assembly of the SWI/SNF complex which is vital for the regulation of transcriptional processes.
Biological function summary

SMARCC1 participates in remodeling chromatin architecture to facilitate or repress gene transcription. It forms an integral part of the SWI/SNF complex which is critical for maintaining proper chromatin structure and function. This complex influences gene expression during developmental processes cell cycle regulation and DNA repair. SMARCC1 interacts with other core components of the SWI/SNF complex such as BAF47 and BRG1 ensuring correct and responsive chromatin modification.

Pathways

SMARCC1 participates in the ATP-dependent chromatin remodeling pathway an important player in gene expression control. This remodeling is connected to the cell cycle pathway as it regulates genes required for cell proliferation. Within these pathways SMARCC1 closely interacts with transcription factors such as c-Myc and other chromatin regulators coordinating the expression of target genes necessary for cell growth and differentiation.

SMARCC1 is linked to cancer and neurodevelopmental disorders. Alterations in SMARCC1 function or expression can lead to dysregulated gene expression contributing to tumorigenesis by affecting pathways involved in cell growth and apoptosis. For instance its association with BRG1 influences pathways implicated in various cancers including solid tumors. Additionally SMARCC1 dysfunction connects to certain neurodevelopmental disorders through the modification of genes critical for brain development and function.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB305038

Anti-SMARCC1/BAF155 antibody [EPR25109-77] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMARCC1/BAF155 antibody [EPR25109-77] - BSA and Azide free (AB305038)

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Product promise

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