Human SMC1B (SMC1L2) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human SMC1B (SMC1L2) knockout HeLa cell lysate (AB258689)
Allele-2 : 1 bp insertion in exon9
- Sanger seq
Unknown
Sanger Sequencing - Human SMC1B (SMC1L2) knockout HeLa cell lysate (AB258689)
Allele-1 : 7 bp deletion in exon9
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays a significant role in ensuring the accurate segregation of chromosomes during both mitosis and meiosis. SMC1L2 is an important component of the cohesin complex interacting closely with other proteins like SMC3 RAD21 and STAG3. By holding sister chromatids together it helps maintain genomic stability and prevents aneuploidy which can lead to compromised cell function or cell death if not regulated properly.
Pathways
SMC1L2 functions in critical cellular mechanisms such as the cell cycle and DNA repair pathways. The protein contributes to the mitotic cell cycle particularly in the regulation of sister chromatid cohesion an essential checkpoint for proper cell cycle progression. Furthermore SMC1L2 involvement in the DNA damage response highlights its connection with proteins like ATM and ATR which act as sensors and mediators of DNA repair mechanisms following genotoxic stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com