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AB263358

Human SMEK2 knockout HeLa cell lysate

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PPP4R3B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.

View Alternative Names

FLFL2, FLJ31474, KIAA1387, PP4R3 beta, PP4R3B, PPP4R3 Beta, PPP4R3B, PSY2, Protein Phosphatase 4, Regulatory Subunit 3 Beta,, SMEK homolog 2, SMEK homolog 2 suppressor of mek1, SMEK homolog 2 suppressor of mek1 (Dictyostelium), Serine/threonine-protein phosphatase 4 regulatory subunit 3B, smk1

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Sanger Sequencing - Human SMEK2 knockout HeLa cell lysate (AB263358)
  • Sanger seq

Unknown

Sanger Sequencing - Human SMEK2 knockout HeLa cell lysate (AB263358)

Homozygous : 2 bp deletion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PPP4R3B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMEK2 also known as protein phosphatase 4 regulatory subunit 3B (PPP4R3B) is a regulatory subunit of the protein phosphatase 4 (PP4) complex. SMEK2 exhibits a molecular mass of about 106 kDa. It is distributed in various tissues with notable expression in the brain and skeletal muscle. As part of the PP4 complex its primary role is to guide and regulate the specific functions of the catalytic subunits of the complex.
Biological function summary

SMEK2 regulates cellular processes by modulating phosphatase activity. It acts as part of the PP4 complex which includes the PP4 catalytic subunit and other regulatory subunits. This complex plays a role in diverse biological functions such as cellular response to DNA damage and cell division. By affecting dephosphorylation events SMEK2 influences cellular signaling allowing for precise cellular responses under varying conditions.

Pathways

SMEK2 significantly impacts the DNA damage response and cell cycle regulation pathways. It interacts closely with proteins such as PP4C the catalytic subunit of the PP4 complex and has connections to key checkpoint regulators in these pathways. Such interactions ensure accurate DNA repair and proper progression through cell cycle phases. Changes in SMEK2’s functionality or expression can disrupt these pathways leading to improper cellular responses.

Altered SMEK2 activity or expression has been associated with cancer and neurodegenerative diseases. In cancer SMEK2 along with PP4C influences tumor suppressor pathways by regulating processes like DNA damage repair. Disruption in these interactions can lead to uncontrolled cell proliferation. Additionally abnormalities in SMEK2 have been linked to Alzheimer's disease possibly due to its involvement in neuronal signaling pathways and response to cellular stress.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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