Human SMIM4 (C3orf78) knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human SMIM4 (C3orf78) knockout HeLa cell lysate (AB259144)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human SMIM4 (C3orf78) knockout HeLa cell lysate (AB259144)
Allele-1 : 1 bp insertion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C3orf78 plays a part in cellular processes that may involve interactions with other proteins as part of a larger complex. Its exact biological role remains under investigation but initial studies suggest it may influence cellular homeostasis and signaling. C3orf78's expression in diverse tissue types implies involvement in multiple physiological functions possibly affecting normal cellular activities through regulatory mechanisms and interactions with yet unidentified partners.
Pathways
Research has positioned C3orf78 within cellular signaling pathways potentially influencing cellular responses. Its connections in the Ras signaling pathway suggest associations with cell growth and differentiation processes. Also C3orf78 could relate to the PI3K-AKT pathway known to be critical for cell survival. Interactions with related signaling proteins such as KRAS and PI3K might affect these pathways highlighting its potential role in modulating cellular events.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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