Human SNAI1 (SNAIL) knockout HeLa cell lysate
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SNAI1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon2 and 68 bp insertion in exon2.
View Alternative Names
Protein sna, Protein snail homolog, Protein snail homolog 1, SLUGH2, SNA, SNAH, SNAI, SNAI1_HUMAN, SNAIL, SNAIL, Drosophila, homolog of, 1, SNAIL1, Sna protein, Snail 1 homolog, Snail 1 zinc finger protein, Snail family transcriptional repressor 1, Snail homolog 1 (Drosophila), Zinc finger protein SNAI1, dJ710H13.1
- WB
Lab
Western blot - Human SNAI1 (SNAIL) knockout HeLa cell lysate (AB257692)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : SNAI1 knockout HeLa cell lysate 20 μg
False colour image of Western blot : Anti-SNAIL antibody [EPR21043] staining at 1/500 dilution, shown in green; loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, ab216347 was shown to bind specifically to SNAIL. A band was observed at 33 kDa in wild-type HeLa cell lysates with no signal observed at this size in SNAI1 knockout cell line ab265963 (knockout cell lysate ab257692). The band observed in the knockout lysate lane below 33 kDa is likely to represent a truncated form of SNAIL. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and SNAI1 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-SNAIL antibody [EPR21043] (<a href='/en-us/products/primary-antibodies/snail-antibody-epr21043-ab216347'>ab216347</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
SNAI1 knockout HeLa cell lysate at 20 µg
Predicted band size: 29 kDa
Observed band size: 33 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SNAI1 (SNAIL) knockout HeLa cell lysate (AB257692)
Allele-1 : 5 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SNAI1 (SNAIL) knockout HeLa cell lysate (AB257692)
Allele-2 : 68 bp insertion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SNAIL contributes to the epithelial-mesenchymal transition (EMT) a process critical for embryogenesis and tumor progression. It forms a part of a complex network of transcription factors that regulate cell-cell adhesion molecules like E-cadherin. SNAIL's ability to bind to specific DNA sequences allows it to suppress or activate the transcription of target genes promoting cellular metamorphosis and enabling cells to acquire motility and invade other tissues.
Pathways
SNAIL operates significantly within the TGF-beta and Wnt signaling pathways. The TGF-beta pathway enhances the expression of SNAIL which in turn represses genes that maintain the epithelial phenotype. The Wnt pathway also modulates SNAIL activity connecting it with proteins such as beta-catenin to drive EMT. These pathways highlight SNAIL's involvement in sophisticated signaling pathways that determine cell behavior adaptation and tissue remodeling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB216347
Anti-SNAIL antibody [EPR21043]
20ul selling size|KO Validated|RabMAb|Recombinant
![Western blot - Anti-SNAIL antibody [EPR21043] (AB216347)](https://content.abcam.com/products/images/snail-antibody-epr21043-ab216347--western-blot-img137742.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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