SNAP25 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 34 bp Deletion in Exon 2.
Images
Key facts
- Cell type
- SH-SY5Y
- Species or organism
- Human
- Tissue
- Bone marrow
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 34 bp Deletion in Exon 2
Alternative names
Bdr, CMS18, FLJ23079, HGNC:11132, MGC105414, MGC139754, RIC-4, Resistance to inhibitors of cholinesterase 4 homolog, SEC 9, SNAP, SNAP-25B, SNP25_HUMAN, SP, SUP, Super protein, Synaptosomal associated protein 25kDa, Synaptosomal-associated 25 kDa protein, Synaptosomal-associated protein, Synaptosomal-associated protein 25, Synaptosomal-associated protein, 25-KD, bA416N4.2, dJ1068F16.2
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SNAP25 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 34 bp Deletion in Exon 2.
Key facts
- Cell type
- SH-SY5Y
- Species or organism
- Human
- Tissue
- Bone marrow
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 34 bp Deletion in Exon 2
- Disease
- Neuroblastoma
- Concentration
Properties
- Gene name
- SNAP25
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAP25 also known as Synaptosomal-associated protein 25 plays a critical role in neurotransmitter release through its involvement in the vesicle fusion process. It is part of the SNARE complex and is essential for the docking and fusion of synaptic vesicles with the presynaptic membrane. The protein is approximately 25 kDa in mass and is expressed abundantly in neurons within the central nervous system. Among its various synonyms you may encounter 'protein SNAP' in some literature.
- Biological function summary
SNARE proteins mediate the fusion of transport vesicles with their target membranes. SNAP25 as part of this complex facilitates synaptic-vesicle exocytosis by forming a tight complex with syntaxin and VAMP (also called synaptobrevin). This interaction promotes the merging of vesicle and plasma membranes enabling efficient neurotransmitter release into the synaptic cleft. SNAP25 undergoes dynamic regulation and modification which is important for precise synaptic function.
- Pathways
One observes SNAP25's integration in the process of neurotransmitter release specifically in the exocytosis pathway. It associates closely with other SNARE proteins such as syntaxin-1 and VAMP-2 embodying a core element in synaptic signaling mechanisms. The proper functioning of SNAP25 within this pathway assures the controlled release of neurotransmitters influencing synaptic plasticity and communication.
- Associated diseases and disorders
SNAP25 exhibits strong associations with conditions such as Attention Deficit Hyperactivity Disorder (ADHD) and epilepsy. Variants and dysregulation in SNAP25 have been linked to problems in synaptic transmission which can contribute to these neurological disorders. The protein also interacts with other SNARE complex proteins such as syntaxin which can potentially mediate its role in disease pathogenesis. Understanding these interactions offers insights into therapeutic targets for neurological disorders involving SNAP25.
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3 product images
Western blot - Human SNAP25 knockout SH-SY5Y cell lysate (ab280100)
Lane 1: Wild-type SH-SY5Y cell lysate 20 μg
Lane 2: SNAP25 knockout SH-SY5Y cell lysate 20 μg
Lane 3: Neuro-2a cell lysate 20 μg
Lane 4: K562 cell lysate 20 μg
False colour image of Western blot: Anti-SNAP25 antibody [EP3274] staining at 1/1000 dilution, shown in green; loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, Anti-SNAP25 antibody [EP3274] ab108990 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line Human SNAP25 knockout SH-SY5Y cell line ab280041 (knockout cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-SNAP25 antibody [EP3274] (Anti-SNAP25 antibody [EP3274] ab108990) at 1/1000 dilution
Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg
Lane 2: SNAP25 knockout SH-SY5Y cell lysate at 20 µg
Lane 2: Western blot - Human SNAP25 knockout SH-SY5Y cell line (Human SNAP25 knockout SH-SY5Y cell line ab280041)
Lane 3: Neuro-2a cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 27 kDa
Western blot - Human SNAP25 knockout SH-SY5Y cell lysate (ab280100)
Lane 1: Wild-type SH-SY5Y cell lysate 20 μg
Lane 2: SNAP25 knockout SH-SY5Y cell lysate 20 μg
Lane 3: Neuro-2a cell lysate 20 μg
Lane 4: K562 cell lysate 20 μg
False colour image of Western blot: Anti-SNAP25 antibody [EPR3275] staining at 1/1000 dilution, shown in green; loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) staining at 1/20000 dilution, shown in red. In Western blot, Anti-SNAP25 antibody [EPR3275] ab109105 was shown to bind specifically to SNAP25. A band was observed at 27 kDa in wild-type SH-SY5Y cell lysates with no signal observed at this size in SNAP25 knockout cell line Human SNAP25 knockout SH-SY5Y cell line ab280041 (knockout cell lysate ab280100). To generate this image, wild-type and SNAP25 knockout SH-SY5Y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.All lanes: Western blot - Anti-SNAP25 antibody [EPR3275] (Anti-SNAP25 antibody [EPR3275] ab109105) at 1/1000 dilution
Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg
Lane 2: SNAP25 knockout SH-SY5Y cell lysate at 20 µg
Lane 2: Western blot - Human SNAP25 knockout SH-SY5Y cell line (Human SNAP25 knockout SH-SY5Y cell line ab280041)
Lane 3: Neuro-2a cell lysate at 20 µg
Lane 4: K562 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 27 kDa
Sanger Sequencing - Human SNAP25 knockout SH-SY5Y cell lysate (ab280100)
Human SNAP25 KO in SH-SY5Y Cells with 34 bp Deletion in Exon 2
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