SNAPIN KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Alternative names
OTTHUMP00000035157, SNAP-25-binding protein, SNAP-associated protein, SNAP25BP, SNAPAP, SNAPN_HUMAN, SNARE-associated protein Snapin, Synaptosomal-associated protein 25-binding protein
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SNAPIN KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- SNAPIN
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SNAPIN also known as SNAP25-interacting protein has an approximate mass of 15 kDa and functions mechanically by interacting with the SNARE complex an important structure in vesicular trafficking. It binds directly to the SNAP25 protein facilitating synaptic vesicle docking and neurotransmitter release at the synapse. Expression of SNAPIN is widespread throughout neuronal tissues and other cell types indicating its role in cellular communication processes.
- Biological function summary
SNAPIN enhances the efficiency of synaptic transmission and vesicle fusion processes. It integrates into the SNARE complex where it stabilizes this multi-protein assembly essential for neurotransmitter release. By bridging interactions between SNAP25 and synaptic vesicles SNAPIN helps bridge essential synapse communication modulating the synaptic transmission needed for effective neuron signaling.
- Pathways
SNAPIN participates in the synaptic vesicle cycle and neurotransmitter release pathways. It works closely in tandem with proteins such as Synaptotagmin and Synaptobrevin which are vital players in the SNARE complex responsible for membrane fusion. By modulating neurotransmitter release SNAPIN contributes to synaptic plasticity and response to neurological stimuli linking it to broader neurological signaling pathways.
- Associated diseases and disorders
Dysfunction of SNAPIN can relate to neurodegenerative diseases including Alzheimer's disease due to its role in neurotransmitter release and synaptic function. Misregulation of SNAPIN function might also implicate it in psychiatric disorders such as schizophrenia where synaptic communication deficits are present. Additionally its involvement with SNAP25 in the SNARE complex associates it with these diseases and potential disruptions in SNARE-associated pathways and synaptic communication.
Product promise
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4 product images
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (ab263359)
Allele-3: 35 bp deletion in exon 1
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (ab263359)
Allele-4: 29 bp deletion in exon 1
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (ab263359)
Allele-2: 50 bp deletion in exon 1
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (ab263359)
Allele-1: Insertion of the selection cassette in exon 1
Downloads
Product protocols
- Visit the general protocols
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