Human SNAPIN knockout HeLa cell lysate
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SNAPIN KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 29 bp deletion in exon 1 and 35 bp deletion in exon 1 and 50 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
OTTHUMP00000035157, SNAP-25-binding protein, SNAP-associated protein, SNAP25BP, SNAPAP, SNAPN_HUMAN, SNARE-associated protein Snapin, Synaptosomal-associated protein 25-binding protein
- Sanger seq
Unknown
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (AB263359)
Allele-3 : 35 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (AB263359)
Allele-4 : 29 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (AB263359)
Allele-2 : 50 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SNAPIN knockout HeLa cell lysate (AB263359)
Allele-1 : Insertion of the selection cassette in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SNAPIN enhances the efficiency of synaptic transmission and vesicle fusion processes. It integrates into the SNARE complex where it stabilizes this multi-protein assembly essential for neurotransmitter release. By bridging interactions between SNAP25 and synaptic vesicles SNAPIN helps bridge essential synapse communication modulating the synaptic transmission needed for effective neuron signaling.
Pathways
SNAPIN participates in the synaptic vesicle cycle and neurotransmitter release pathways. It works closely in tandem with proteins such as Synaptotagmin and Synaptobrevin which are vital players in the SNARE complex responsible for membrane fusion. By modulating neurotransmitter release SNAPIN contributes to synaptic plasticity and response to neurological stimuli linking it to broader neurological signaling pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB267503
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RabMAb|Recombinant
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- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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