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AB257694

Human SNCG (gamma Synuclein) knockout HeLa cell lysate

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SNCG KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

View Alternative Names

BCSG1, Breast cancer specific protein 1, Breast cancer-specific gene 1 protein, Gamma-synuclein, PRSN, Persyn, SNCG, SR, SYUG_HUMAN, Synoretin, Synuclein gamma, synuclein, gamma (breast cancer-specific protein 1)

3 Images
Western blot - Human SNCG (gamma Synuclein) knockout HeLa cell lysate (AB257694)
  • WB

Lab

Western blot - Human SNCG (gamma Synuclein) knockout HeLa cell lysate (AB257694)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : SNCG knockout HeLa cell lysate (20 ug)
Lane 3 : SK-BR-3 cell lysate (20 ug)

ab52633 was shown to specifically react with gamma Synuclein/SNCG antibody in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265623 (knockout cell lysate ab257694) was used. Wild-type and gamma Synuclein/SNCG knockout samples were subjected to SDS-PAGE. ab52633 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-gamma Synuclein/SNCG antibody [EP1539Y] (<a href='/en-us/products/primary-antibodies/gamma-synuclein-sncg-antibody-ep1539y-ab52633'>ab52633</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SNCG knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SNCG (gamma Synuclein) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-sncg-gamma-synuclein-knockout-hela-cell-line-ab265623'>ab265623</a>)

Lane 3:

SK-BR-3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 13 kDa

Observed band size: 16 kDa

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Sanger Sequencing - Human SNCG (gamma Synuclein) knockout HeLa cell lysate (AB257694)
  • Sanger seq

Unknown

Sanger Sequencing - Human SNCG (gamma Synuclein) knockout HeLa cell lysate (AB257694)

Allele-1 : 1 bp deletion in exon 1

Sanger Sequencing - Human SNCG (gamma Synuclein) knockout HeLa cell lysate (AB257694)
  • Sanger seq

Unknown

Sanger Sequencing - Human SNCG (gamma Synuclein) knockout HeLa cell lysate (AB257694)

Allele-2 : Insertion of the selection cassette in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

Disease

Adenocarcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
SNCG
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Gamma synuclein sometimes referred to as SNCG or gamma-synuclein is a protein with a molecular weight of approximately 13 kDa. It is a member of the synuclein family which also includes alpha and beta synucleins. This protein is highly expressed in the nervous system particularly in the peripheral nervous system and certain regions of the central nervous system. Outside the nervous system gamma synuclein can be found in the retina reproductive tissues and breast tissue among others.
Biological function summary

Gamma synuclein plays a role in modulating neuronal plasticity and may participate in synaptic regulation. It is not known to be a part of a larger protein complex but it interacts with various other proteins influencing neuron differentiation. Although its precise biological functions are not fully characterized studies suggest that gamma synuclein could impact cellular processes like cytoskeletal dynamics and membrane trafficking.

Pathways

The protein gamma synuclein is involved in cellular stress response and neurodegeneration pathways. It is closely related to proteins like alpha synuclein with which it shares structural and functional features. Pathways involving these proteins can influence several processes such as axonal transport and neurotransmitter release. Dysregulation in these pathways often leads to disruptions in cellular homeostasis.

The involvement of gamma synuclein is most notably seen in breast cancer and neurodegenerative diseases such as Alzheimer's disease. Researchers have observed that this protein is overexpressed in various tumors where it might facilitate cancer progression and metastasis. In neurodegeneration interaction with alpha synuclein and tau protein could play a role in the pathogenesis where protein aggregation is a characteristic feature. Understanding its role in these diseases helps in charting possible therapeutic avenues.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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