Human SNRK knockout HeLa cell lysate
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SNRK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon6 and 1 bp deletion in exon6.
View Alternative Names
DKFZp779A1866, FLJ20224, HSNFRK, KIAA0096, SNF related kinase, SNF-related serine/threonine-protein kinase, SNF1-related kinase, SNFRK, SNRK_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human SNRK knockout HeLa cell lysate (AB259145)
Allele-2 : 1 bp deletion in exon6
- Sanger seq
Unknown
Sanger Sequencing - Human SNRK knockout HeLa cell lysate (AB259145)
Allele-1 : 11 bp deletion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Through phosphorylation SNRK controls energy balance and cellular stress responses. It does not function as part of a large protein complex but can interact with other proteins to modulate metabolic processes. SNRK contributes to regulating cell growth apoptosis and metabolism. It has a significant role in maintaining energy homeostasis and responding to nutrient availability potentially affecting metabolic syndrome.
Pathways
SNRK participates in energy-sensing pathways. It plays an important role alongside AMP-activated protein kinase (AMPK) involved in response to cellular energy levels. SNRK is also linked to the insulin signaling pathway which regulates glucose metabolism. Its activity impacts downstream targets such as acetyl-CoA carboxylase an important enzyme related to fatty acid metabolism. This indicates SNRK's importance in pathways influencing metabolic health and organism energy status.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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