Human SNRNP40 knockout HEK-293T cell lysate
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SNRNP40 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 8 bp deletion in exon 1.
View Alternative Names
38 kDa-splicing factor, 40K, FLJ41108, MGC1910, PRP8BP, PRPF8BP, Prp8-binding protein, RP11-490K7.3, SNR40_HUMAN, SPF38, U5 small nuclear ribonucleoprotein 40 kDa protein, U5 snRNP 40 kDa protein, U5 snRNP-specific 40 kDa protein, U5 snRNP-specific 40 kDa protein (hPrp8-binding), U5-40K, WD repeat domain 57 (U5 snRNP specific), WD repeat-containing protein 57, WDR57, hPRP8BP, small nuclear ribonucleoprotein 40kDa (U5)
- Sanger seq
Unknown
Sanger Sequencing - Human SNRNP40 knockout HEK-293T cell lysate (AB259146)
Allele-1 : 8 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human SNRNP40 knockout HEK-293T cell lysate (AB259146)
Allele-2 : 1 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SNRNP40 participates in the pre-mRNA splicing process as part of the spliceosome a large and dynamic ribonucleoprotein complex. The spliceosome facilitates the removal of introns from pre-mRNA transcripts. SNRNP40 as a part of this complex influences the conformational changes necessary for the accurate assembly and function of the spliceosome. Through this action SNRNP40 helps maintain genomic stability and proper gene expression.
Pathways
SNRNP40's role in the spliceosome places it within essential RNA processing pathways including the mRNA splicing pathway. It interacts with other proteins such as PRPF8 and EFTUD2 (also known as Snu114) which are key components of the U5 particle. These interactions illustrate how SNRNP40's activity is integral to the functioning of the spliceosomal machinery.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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