Human SOD2 (MnSOD) knockout HEK-293T cell lysate
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SOD2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
IPO B, Indophenoloxidase B, MVCD6, Manganese SOD, Manganese containing superoxide dismutase, Manganese superoxide dismutase, Mangano superoxide dismutase, Mn SOD, Mn superoxide dismutase, SOD 2, SODM_HUMAN, Superoxide dismutase 2 mitochondrial, Superoxide dismutase [Mn] mitochondrial
- WB
Lab
Western blot - Human SOD2 (MnSOD) knockout HEK-293T cell lysate (AB263835)
Lane 1 : SH-SY5Y cell lysate (20 μg)
Lane 2 : A549 cell lysate (20 μg)
Lane 3 : Wild-type HEK-293T cell lysate (20 μg)
Lane 4 : SOD2 knockout HEK-293T cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab68155 observed at 22 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab68155 was shown to react with SOD2/MnSOD in wild-type HEK-293T. Loss of signal was observed when knockout cell line ab255434 (knockout cell lysate ab263835) was used. Wild-type and SOD2/MnSOD knockout samples were subjected to SDS-PAGE. ab68155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (<a href='/en-us/products/primary-antibodies/sod2-mnsod-antibody-epr2560y-ab68155'>ab68155</a>) at 1/1000 dilution
Lane 1:
SHSY5Y cell lysate at 20 µg
Lane 2:
A549 cell lysate at 20 µg
Lane 2:
Western blot - Human SOD2 (MnSOD) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-sod2-mnsod-knockout-hek-293t-cell-line-ab255434'>ab255434</a>)
Lane 3:
Wild-type HEK-293T cell lysate at 20 µg
Lane 4:
SOD2 knockout HEK-293T cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 24 kDa
Observed band size: 29 kDa,37 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human SOD2 (MnSOD) knockout HEK-293T cell lysate (AB263835)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human SOD2 (MnSOD) knockout HEK-293T cell lysate (AB263835)
Allele-1 : 16 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-SOD2/MnSOD antibody [EPR2560Y] (AB68155)](https://content.abcam.com/products/images/sod2-mnsod-antibody-epr2560y-ab68155--western-blot-img152739.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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